% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Wehnekamp:140759,
      author       = {Wehnekamp, Fabian and Plucińska, Gabriela and Thong,
                      Rachel and Misgeld, Thomas and Lamb, Don C},
      title        = {{N}anoresolution real-time 3{D} orbital tracking for
                      studying mitochondrial trafficking in vertebrate axons in
                      vivo.},
      journal      = {eLife},
      volume       = {8},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DZNE-2020-07081},
      pages        = {e46059},
      year         = {2019},
      abstract     = {We present the development and in vivo application of a
                      feedback-based tracking microscope to follow individual
                      mitochondria in sensory neurons of zebrafish larvae with
                      nanometer precision and millisecond temporal resolution. By
                      combining various technical improvements, we tracked
                      individual mitochondria with unprecedented spatiotemporal
                      resolution over distances of >100 µm. Using these
                      nanoscopic trajectory data, we discriminated five motional
                      states: a fast and a slow directional motion state in both
                      the anterograde and retrograde directions and a stationary
                      state. The transition pattern revealed that, after a pause,
                      mitochondria predominantly persist in the original direction
                      of travel, while transient changes of direction often
                      exhibited longer pauses. Moreover, mitochondria in the
                      vicinity of a second, stationary mitochondria displayed an
                      increased probability to pause. The capability of following
                      and optically manipulating a single organelle with high
                      spatiotemporal resolution in a living organism offers a new
                      approach to elucidating their function in its complete
                      physiological context.},
      keywords     = {Animals / Axons: metabolism / Biological Transport / Cell
                      Tracking: methods / Imaging, Three-Dimensional: methods /
                      Larva: metabolism / Microscopy, Confocal: methods /
                      Mitochondria: metabolism / Nanotechnology: methods / Sensory
                      Receptor Cells: metabolism / Vertebrates: metabolism /
                      Zebrafish: metabolism},
      cin          = {München common / AG Misgeld},
      ddc          = {600},
      cid          = {I:(DE-2719)6000016 / I:(DE-2719)1110000-4},
      pnm          = {341 - Molecular Signaling (POF3-341)},
      pid          = {G:(DE-HGF)POF3-341},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31180320},
      pmc          = {pmc:PMC6579510},
      doi          = {10.7554/eLife.46059},
      url          = {https://pub.dzne.de/record/140759},
}