Journal Article

DZNE-2020-07081

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo.

Wehnekamp, F. ; Plucińska, G.DZNE* ; Thong, R.DZNE* ; Misgeld, T. (Corresponding author)DZNE* ; Lamb, D. C.

2019
eLife Sciences Publications Cambridge

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Please use a persistent id in citations: doi:10.7554/eLife.46059

Abstract: We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unprecedented spatiotemporal resolution over distances of >100 µm. Using these nanoscopic trajectory data, we discriminated five motional states: a fast and a slow directional motion state in both the anterograde and retrograde directions and a stationary state. The transition pattern revealed that, after a pause, mitochondria predominantly persist in the original direction of travel, while transient changes of direction often exhibited longer pauses. Moreover, mitochondria in the vicinity of a second, stationary mitochondria displayed an increased probability to pause. The capability of following and optically manipulating a single organelle with high spatiotemporal resolution in a living organism offers a new approach to elucidating their function in its complete physiological context.

Keyword(s): Animals (MeSH) ; Axons: metabolism (MeSH) ; Biological Transport (MeSH) ; Cell Tracking: methods (MeSH) ; Imaging, Three-Dimensional: methods (MeSH) ; Larva: metabolism (MeSH) ; Microscopy, Confocal: methods (MeSH) ; Mitochondria: metabolism (MeSH) ; Nanotechnology: methods (MeSH) ; Sensory Receptor Cells: metabolism (MeSH) ; Vertebrates: metabolism (MeSH) ; Zebrafish: metabolism (MeSH)

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Contributing Institute(s):

1. München common (München common)
2. Neuronal Cell Biology (AG Misgeld)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2019

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Database coverage:
; ; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; IF >= 5 ; JCR ; SCOPUS ; Web of Science Core Collection ; Zoological Record

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Mieskes, F. ; Wehnekamp, F. ; Plucińska, G.DZNE* ; Thong, R.DZNE* ; Misgeld, T.DZNE* ; Lamb, D. C.
Trajectory data of antero- and retrograde movement of mitochondria in living zebrafish larvae.
Data in Brief 29, 105280 (2020) [10.1016/j.dib.2020.105280]2020   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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