guest ::
| Home > Publications Database > Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo. > print |
| Home > Publications Database > Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo. > print |
| 001 | 140759 | ||
| 005 | 20240430120012.0 | ||
| 024 | 7 | _ | |a 10.7554/eLife.46059 |2 doi |
| 024 | 7 | _ | |a pmid:31180320 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC6579510 |2 pmc |
| 024 | 7 | _ | |a altmetric:61882442 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-07081 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 600 |
| 100 | 1 | _ | |a Wehnekamp, Fabian |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo. |
| 260 | _ | _ | |a Cambridge |c 2019 |b eLife Sciences Publications |
| 264 | _ | 1 | |3 online |2 Crossref |b eLife Sciences Publications, Ltd |c 2019-06-10 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1714462136_8222 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unprecedented spatiotemporal resolution over distances of >100 µm. Using these nanoscopic trajectory data, we discriminated five motional states: a fast and a slow directional motion state in both the anterograde and retrograde directions and a stationary state. The transition pattern revealed that, after a pause, mitochondria predominantly persist in the original direction of travel, while transient changes of direction often exhibited longer pauses. Moreover, mitochondria in the vicinity of a second, stationary mitochondria displayed an increased probability to pause. The capability of following and optically manipulating a single organelle with high spatiotemporal resolution in a living organism offers a new approach to elucidating their function in its complete physiological context. |
| 536 | _ | _ | |a 341 - Molecular Signaling (POF3-341) |0 G:(DE-HGF)POF3-341 |c POF3-341 |f POF III |x 0 |
| 542 | _ | _ | |i 2019-06-10 |2 Crossref |u http://creativecommons.org/licenses/by/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Axons: metabolism |2 MeSH |
| 650 | _ | 2 | |a Biological Transport |2 MeSH |
| 650 | _ | 2 | |a Cell Tracking: methods |2 MeSH |
| 650 | _ | 2 | |a Imaging, Three-Dimensional: methods |2 MeSH |
| 650 | _ | 2 | |a Larva: metabolism |2 MeSH |
| 650 | _ | 2 | |a Microscopy, Confocal: methods |2 MeSH |
| 650 | _ | 2 | |a Mitochondria: metabolism |2 MeSH |
| 650 | _ | 2 | |a Nanotechnology: methods |2 MeSH |
| 650 | _ | 2 | |a Sensory Receptor Cells: metabolism |2 MeSH |
| 650 | _ | 2 | |a Vertebrates: metabolism |2 MeSH |
| 650 | _ | 2 | |a Zebrafish: metabolism |2 MeSH |
| 700 | 1 | _ | |a Plucińska, Gabriela |0 P:(DE-2719)9000910 |b 1 |u dzne |
| 700 | 1 | _ | |a Thong, Rachel |0 P:(DE-2719)9000912 |b 2 |u dzne |
| 700 | 1 | _ | |a Misgeld, Thomas |0 P:(DE-2719)2810727 |b 3 |e Corresponding author |u dzne |
| 700 | 1 | _ | |a Lamb, Don C |0 P:(DE-HGF)0 |b 4 |
| 773 | 1 | 8 | |a 10.7554/elife.46059 |b : eLife Sciences Publications, Ltd, 2019-06-10 |3 journal-article |2 Crossref |t eLife |v 8 |y 2019 |x 2050-084X |
| 773 | _ | _ | |a 10.7554/eLife.46059 |g Vol. 8, p. e46059 |0 PERI:(DE-600)2687154-3 |q 8 |t eLife |v 8 |y 2019 |x 2050-084X |
| 856 | 4 | _ | |y OpenAccess |u https://pub.dzne.de/record/140759/files/DZNE-2020-07081.pdf |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://pub.dzne.de/record/140759/files/DZNE-2020-07081.pdf?subformat=pdfa |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6579510 |
| 909 | C | O | |o oai:pub.dzne.de:140759 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 1 |6 P:(DE-2719)9000910 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 2 |6 P:(DE-2719)9000912 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 3 |6 P:(DE-2719)2810727 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-341 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Molecular Signaling |x 0 |
| 914 | 1 | _ | |y 2019 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-23 |
| 915 | _ | _ | |a Creative Commons Attribution CC BY (No Version) |0 LIC:(DE-HGF)CCBYNV |2 V:(DE-HGF) |b DOAJ |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record |d 2022-11-23 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b ELIFE : 2021 |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |d 2022-09-23T12:20:44Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |d 2022-09-23T12:20:44Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2022-11-23 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |d 2022-11-23 |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b ELIFE : 2021 |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-23 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2022-11-23 |
| 920 | 1 | _ | |0 I:(DE-2719)6000016 |k München common |l München common |x 0 |
| 920 | 1 | _ | |0 I:(DE-2719)1110000-4 |k AG Misgeld |l Neuronal Cell Biology |x 1 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-2719)6000016 |
| 980 | _ | _ | |a I:(DE-2719)1110000-4 |
| 980 | 1 | _ | |a FullTexts |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1083/jcb.201401070 |2 Crossref |o 10.1083/jcb.201401070 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/cdd.2012.81 |2 Crossref |o 10.1038/cdd.2012.81 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/0960-9822(92)90896-I |2 Crossref |o 10.1016/0960-9822(92)90896-I |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1073/pnas.1219206110 |2 Crossref |o 10.1073/pnas.1219206110 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.1556-10.2010 |2 Crossref |o 10.1523/JNEUROSCI.1556-10.2010 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cell.2015.06.049 |2 Crossref |o 10.1016/j.cell.2015.06.049 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nm.3520 |2 Crossref |o 10.1038/nm.3520 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.pneurobio.2006.09.003 |2 Crossref |o 10.1016/j.pneurobio.2006.09.003 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nprot.2007.291 |2 Crossref |o 10.1038/nprot.2007.291 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nrn2631 |2 Crossref |o 10.1038/nrn2631 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cell.2013.05.021 |2 Crossref |o 10.1016/j.cell.2013.05.021 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.1198-15.2015 |2 Crossref |o 10.1523/JNEUROSCI.1198-15.2015 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1088/1367-2630/15/7/075008 |2 Crossref |o 10.1088/1367-2630/15/7/075008 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.7554/eLife.11583 |2 Crossref |o 10.7554/eLife.11583 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1093/hmg/dds190 |2 Crossref |o 10.1093/hmg/dds190 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1101/pdb.top119 |2 Crossref |o 10.1101/pdb.top119 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/s41556-019-0317-2 |2 Crossref |o 10.1038/s41556-019-0317-2 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth818 |2 Crossref |o 10.1038/nmeth818 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nrm2774 |2 Crossref |o 10.1038/nrm2774 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.neuron.2010.09.039 |2 Crossref |o 10.1016/j.neuron.2010.09.039 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1021/nl1028792 |2 Crossref |o 10.1021/nl1028792 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/S0006-3495(94)80601-0 |2 Crossref |o 10.1016/S0006-3495(94)80601-0 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1002/cphc.200900436 |2 Crossref |o 10.1002/cphc.200900436 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1002/aja.1002030302 |2 Crossref |o 10.1002/aja.1002030302 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/71338 |2 Crossref |o 10.1038/71338 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1006/dbio.2001.0242 |2 Crossref |o 10.1006/dbio.2001.0242 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/0955-0674(95)80048-4 |2 Crossref |o 10.1016/0955-0674(95)80048-4 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1002/jbio.201100002 |2 Crossref |o 10.1002/jbio.201100002 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1063/1.2819074 |2 Crossref |o 10.1063/1.2819074 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1529/biophysj.104.044230 |2 Crossref |o 10.1529/biophysj.104.044230 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cub.2016.07.064 |2 Crossref |o 10.1016/j.cub.2016.07.064 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1002/1096-9861(20001120)427:3<340::AID-CNE2>3.0.CO;2-Y |2 Crossref |o 10.1002/1096-9861(20001120)427:3<340::AID-CNE2>3.0.CO;2-Y |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.tcb.2009.11.002 |2 Crossref |o 10.1016/j.tcb.2009.11.002 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nature02293 |2 Crossref |o 10.1038/nature02293 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1021/nl0723376 |2 Crossref |o 10.1021/nl0723376 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/B978-0-12-391857-4.00009-4 |2 Crossref |o 10.1016/B978-0-12-391857-4.00009-4 |
| 999 | C | 5 | |y 2016 |2 Crossref |t Cell Biology by the Numbers |o Milo Cell Biology by the Numbers 2016 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth1055 |2 Crossref |o 10.1038/nmeth1055 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.neuron.2017.09.055 |2 Crossref |o 10.1016/j.neuron.2017.09.055 |
| 999 | C | 5 | |y 1993 |2 Crossref |o Morris 1993 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1083/jcb.131.5.1315 |2 Crossref |o 10.1083/jcb.131.5.1315 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/S0960-9822(00)00048-8 |2 Crossref |o 10.1016/S0960-9822(00)00048-8 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.1331-13.2013 |2 Crossref |o 10.1523/JNEUROSCI.1331-13.2013 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.jtbi.2008.09.009 |2 Crossref |o 10.1016/j.jtbi.2008.09.009 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1111/ejn.12263 |2 Crossref |o 10.1111/ejn.12263 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.0095-11.2011 |2 Crossref |o 10.1523/JNEUROSCI.0095-11.2011 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.1621-10.2010 |2 Crossref |o 10.1523/JNEUROSCI.1621-10.2010 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cell.2014.06.007 |2 Crossref |o 10.1016/j.cell.2014.06.007 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/ncomms8874 |2 Crossref |o 10.1038/ncomms8874 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.1327-12.2012 |2 Crossref |o 10.1523/JNEUROSCI.1327-12.2012 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.conb.2016.06.006 |2 Crossref |o 10.1016/j.conb.2016.06.006 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/S0042-6989(01)00284-X |2 Crossref |o 10.1016/S0042-6989(01)00284-X |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nn.4161 |2 Crossref |o 10.1038/nn.4161 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1371/journal.pbio.1001754 |2 Crossref |o 10.1371/journal.pbio.1001754 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1242/jcs.053850 |2 Crossref |o 10.1242/jcs.053850 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1021/acs.nanolett.5b01396 |2 Crossref |o 10.1021/acs.nanolett.5b01396 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1083/jcb.201312123 |2 Crossref |o 10.1083/jcb.201312123 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nrn3156 |2 Crossref |o 10.1038/nrn3156 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cub.2016.07.033 |2 Crossref |o 10.1016/j.cub.2016.07.033 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.celrep.2013.11.022 |2 Crossref |o 10.1016/j.celrep.2013.11.022 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1093/nar/gku1194 |2 Crossref |o 10.1093/nar/gku1194 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1073/pnas.0508511103 |2 Crossref |o 10.1073/pnas.0508511103 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nature14128 |2 Crossref |o 10.1038/nature14128 |
| 999 | C | 5 | |y 2015 |2 Crossref |t GitLab |o Wehnekamp GitLab 2015 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nnano.2014.12 |2 Crossref |o 10.1038/nnano.2014.12 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|