TY  - JOUR
AU  - Knauer, Beate
AU  - Yoshida, Motoharu
TI  - Switching between persistent firing and depolarization block in individual rat CA1 pyramidal neurons.
JO  - Hippocampus
VL  - 29
IS  - 9
SN  - 1050-9631
CY  - New York, NY [u.a.]
PB  - Wiley
M1  - DZNE-2020-07206
SP  - 817-835
PY  - 2019
AB  - The hippocampal formation plays a role in mnemonic tasks and epileptic discharges in vivo. In vitro, these functions and malfunctions may relate to persistent firing (PF) and depolarization block (DB), respectively. Pyramidal neurons of the CA1 field have previously been reported to engage in either PF or DB during cholinergic stimulation. However, it is unknown whether these cells constitute disparate populations of neurons. Furthermore, it is unclear which cell-specific peculiarities may mediate their diverse response properties. However, it has not been shown whether individual CA1 pyramidal neurons can switch between PF and DB states. Here, we used whole cell patch clamp in the current clamp mode on in vitro CA1 pyramidal neurons from acutely sliced rat tissue to test various intrinsic properties which may provoke individual cells to switch between PF and DB. We found that individual cells could switch from PF to DB, in a cholinergic agonist concentration dependent manner and depending on the parameters of stimulation. We also demonstrate involvement of TRPC and potassium channels in this switching. Finally, we report that the probability for DB was more pronounced in the proximal than in the distal half of CA1. These findings offer a potential mechanism for the stronger spatial modulation in proximal, compared to distal CA1, as place field formation was shown to be affected by DB. Taken together, our results suggest that PF and DB are not mutually exclusive response properties of individual neurons. Rather, a cell's response mode depends on a variety of intrinsic properties, and modulation of these properties enables switching between PF and DB.
KW  - Animals
KW  - CA1 Region, Hippocampal: cytology
KW  - CA1 Region, Hippocampal: drug effects
KW  - CA1 Region, Hippocampal: physiology
KW  - Carbachol: pharmacology
KW  - Dose-Response Relationship, Drug
KW  - Electrophysiological Phenomena: drug effects
KW  - Electrophysiological Phenomena: physiology
KW  - Female
KW  - In Vitro Techniques
KW  - Male
KW  - Membrane Potentials: drug effects
KW  - Muscarinic Agonists: pharmacology
KW  - Patch-Clamp Techniques
KW  - Potassium Channels: metabolism
KW  - Pyramidal Cells: drug effects
KW  - Pyramidal Cells: physiology
KW  - Rats
KW  - Rats, Long-Evans
KW  - TRPC Cation Channels: antagonists & inhibitors
KW  - TRPC Cation Channels: metabolism
LB  - PUB:(DE-HGF)16
C6  - pmid:30794330
DO  - DOI:10.1002/hipo.23078
UR  - https://pub.dzne.de/record/140884
ER  -