Journal Article

DZNE-2020-07206

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Switching between persistent firing and depolarization block in individual rat CA1 pyramidal neurons.

Knauer, B. ; Yoshida, M. (Last author)DZNE*

2019
Wiley New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1002/hipo.23078

Abstract: The hippocampal formation plays a role in mnemonic tasks and epileptic discharges in vivo. In vitro, these functions and malfunctions may relate to persistent firing (PF) and depolarization block (DB), respectively. Pyramidal neurons of the CA1 field have previously been reported to engage in either PF or DB during cholinergic stimulation. However, it is unknown whether these cells constitute disparate populations of neurons. Furthermore, it is unclear which cell-specific peculiarities may mediate their diverse response properties. However, it has not been shown whether individual CA1 pyramidal neurons can switch between PF and DB states. Here, we used whole cell patch clamp in the current clamp mode on in vitro CA1 pyramidal neurons from acutely sliced rat tissue to test various intrinsic properties which may provoke individual cells to switch between PF and DB. We found that individual cells could switch from PF to DB, in a cholinergic agonist concentration dependent manner and depending on the parameters of stimulation. We also demonstrate involvement of TRPC and potassium channels in this switching. Finally, we report that the probability for DB was more pronounced in the proximal than in the distal half of CA1. These findings offer a potential mechanism for the stronger spatial modulation in proximal, compared to distal CA1, as place field formation was shown to be affected by DB. Taken together, our results suggest that PF and DB are not mutually exclusive response properties of individual neurons. Rather, a cell's response mode depends on a variety of intrinsic properties, and modulation of these properties enables switching between PF and DB.

Keyword(s): Animals (MeSH) ; CA1 Region, Hippocampal: cytology (MeSH) ; CA1 Region, Hippocampal: drug effects (MeSH) ; CA1 Region, Hippocampal: physiology (MeSH) ; Carbachol: pharmacology (MeSH) ; Dose-Response Relationship, Drug (MeSH) ; Electrophysiological Phenomena: drug effects (MeSH) ; Electrophysiological Phenomena: physiology (MeSH) ; Female (MeSH) ; In Vitro Techniques (MeSH) ; Male (MeSH) ; Membrane Potentials: drug effects (MeSH) ; Muscarinic Agonists: pharmacology (MeSH) ; Patch-Clamp Techniques (MeSH) ; Potassium Channels: metabolism (MeSH) ; Pyramidal Cells: drug effects (MeSH) ; Pyramidal Cells: physiology (MeSH) ; Rats (MeSH) ; Rats, Long-Evans (MeSH) ; TRPC Cation Channels: antagonists & inhibitors (MeSH) ; TRPC Cation Channels: metabolism (MeSH)

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Contributing Institute(s):

1. Cognitive Neurophysiology (AG Yoshida)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2019

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Web of Science Core Collection

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