TY  - JOUR
AU  - Leiter, Odette
AU  - Bernas, Stefanie N
AU  - Seidemann, Suse
AU  - Overall, Rupert W
AU  - Horenburg, Cindy
AU  - Kowal, Susann
AU  - Kempermann, Gerd
AU  - Walker, Tara
TI  - The systemic exercise-released chemokine lymphotactin/XCL1 modulates in vitro adult hippocampal precursor cell proliferation and neuronal differentiation.
JO  - Scientific reports
VL  - 9
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DZNE-2020-07208
SP  - 11831
PY  - 2019
AB  - Physical exercise has well-established anti-inflammatory effects, with neuro-immunological crosstalk being proposed as a mechanism underlying the beneficial effects of exercise on brain health. Here, we used physical exercise, a strong positive modulator of adult hippocampal neurogenesis, as a model to identify immune molecules that are secreted into the blood stream, which could potentially mediate this process. Proteomic profiling of mouse plasma showed that levels of the chemokine lymphotactin (XCL1) were elevated after four days of running. We found that XCL1 treatment of primary cells isolated from both the dentate gyrus and the subventricular zone of the adult mice led to an increase in the number of neurospheres and neuronal differentiation in neurospheres derived from the dentate gyrus. In contrast, primary dentate gyrus cells isolated from XCL1 knockout mice formed fewer neurospheres and exhibited a reduced neuronal differentiation potential. XCL1 supplementation in a dentate gyrus-derived neural precursor cell line promoted neuronal differentiation and resulted in lower cell motility and a reduced number of cells in the S phase of the cell cycle. This work suggests an additional function of the chemokine XCL1 in the brain and underpins the complexity of neuro-immune interactions that contribute to the regulation of adult hippocampal neurogenesis.
KW  - Animals
KW  - Cell Differentiation
KW  - Cell Proliferation
KW  - Chemokines, C: metabolism
KW  - Hippocampus: cytology
KW  - Hippocampus: metabolism
KW  - In Vitro Techniques
KW  - Mice
KW  - Mice, Knockout
KW  - Neurons: cytology
KW  - Physical Conditioning, Animal
LB  - PUB:(DE-HGF)16
C6  - pmid:31413355
C2  - pmc:PMC6694144
DO  - DOI:10.1038/s41598-019-48360-5
UR  - https://pub.dzne.de/record/140886
ER  -