%0 Journal Article
%A Semyanov, Alexey
%A Henneberger, Christian
%A Agarwal, Amit
%T Making sense of astrocytic calcium signals — from acquisition to interpretation
%J Nature reviews / Neuroscience
%V 21
%N 10
%@ 1471-0048
%C London
%I Nature Publ. Group
%M DZNE-2020-01434
%P 551 - 564
%D 2020
%X Astrocytes functionally interact with neurons and with other brain cells. Although not electrically excitable, astrocytes display a complex repertoire of intracellular Ca2+ signalling that evolves in space and time within single astrocytes and across astrocytic networks. Decoding the physiological meaning of these dynamic changes in astrocytic Ca2+ activity has remained a major challenge. This Review describes experimental preparations and methods for recording and studying Ca2+ activity in astrocytes, focusing on the analysis of Ca2+ signalling events in single astrocytes and in astrocytic networks. The limitations of existing experimental approaches and ongoing technical and conceptual challenges in the interpretation of astrocytic Ca2+ events and their spatio-temporal patterns are also discussed.
%K Animals
%K Astrocytes: physiology
%K Brain: physiology
%K Calcium Signaling: physiology
%K Cells, Cultured
%K Humans
%K Neurons: physiology
%K Optical Imaging
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:32873937
%R 10.1038/s41583-020-0361-8
%U https://pub.dzne.de/record/153437