TY  - JOUR
AU  - Semyanov, Alexey
AU  - Henneberger, Christian
AU  - Agarwal, Amit
TI  - Making sense of astrocytic calcium signals — from acquisition to interpretation
JO  - Nature reviews / Neuroscience
VL  - 21
IS  - 10
SN  - 1471-0048
CY  - London
PB  - Nature Publ. Group
M1  - DZNE-2020-01434
SP  - 551 - 564
PY  - 2020
AB  - Astrocytes functionally interact with neurons and with other brain cells. Although not electrically excitable, astrocytes display a complex repertoire of intracellular Ca2+ signalling that evolves in space and time within single astrocytes and across astrocytic networks. Decoding the physiological meaning of these dynamic changes in astrocytic Ca2+ activity has remained a major challenge. This Review describes experimental preparations and methods for recording and studying Ca2+ activity in astrocytes, focusing on the analysis of Ca2+ signalling events in single astrocytes and in astrocytic networks. The limitations of existing experimental approaches and ongoing technical and conceptual challenges in the interpretation of astrocytic Ca2+ events and their spatio-temporal patterns are also discussed.
KW  - Animals
KW  - Astrocytes: physiology
KW  - Brain: physiology
KW  - Calcium Signaling: physiology
KW  - Cells, Cultured
KW  - Humans
KW  - Neurons: physiology
KW  - Optical Imaging
LB  - PUB:(DE-HGF)16
C6  - pmid:32873937
DO  - DOI:10.1038/s41583-020-0361-8
UR  - https://pub.dzne.de/record/153437
ER  -