Journal Article

DZNE-2021-00880

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Differential expression of gut miRNAs in idiopathic Parkinson's disease.

Kurz, A. (First author)DZNE* ; Kumar, R.DZNE* ; Northoff, B. H. ; Wenk, C. ; Schirra, J. ; Donakonda, S. ; Höglinger, G. U.DZNE* ; Schwarz, J.Extern* ; Rozanski, V. ; Hübner, R. ; Bötzel, K. ; Holdt, L. M. ; Koeglsperger, T. (Last author)DZNE*

2021
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.parkreldis.2021.05.022

Abstract: In the present work, we aimed to investigate the expression of microRNAs (miRNAs) in routine colonic biopsies obtained from patients with idiopathic Parkinson's disease (PD) and to address their value as a diagnostic biomarker for PD and their mechanistic contribution to PD onset and progression.Patients with PD (n = 13) and healthy controls (n = 17) were prospectively recruited to undergo routine colonic biopsies for cancer screening. Total RNA was extracted from the biopsy material and the expression of miRNAs was quantified by Illumina High-Throughput Sequencing.Statistical analysis revealed a significant submucosal enrichment of the miRNA hsa-miR-486-5p in colonic biopsies from PD patients compared to the control subjects. The expression of miR-486-5p correlated with age and disease severity as measured by the UPDRS and Hoehn & Yahr scale. miRNA gene target analysis identified 301 gene targets that are affected by miR-486-5p. A follow-up associated target identification and pathway enrichment analysis further determined their role in distinct biological processes in the enteric nervous system (ENS).Our work demonstrates an enrichment of submucosal miR-486-5p in routine colonic biopsies from PD patients. Our results will support the examination of miR-486-5p as a PD biomarker and help to understand the significance of the miR-486-5p gene targets for PD onset and progression. In addition, our data will support the investigation of the molecular and cellular mechanisms of GI dysfunction in PD.

Keyword(s): Age Factors (MeSH) ; Aged (MeSH) ; Biomarkers: metabolism (MeSH) ; Biopsy (MeSH) ; Colon: metabolism (MeSH) ; Colon: pathology (MeSH) ; Enteric Nervous System: metabolism (MeSH) ; Female (MeSH) ; Gene Expression Profiling (MeSH) ; Humans (MeSH) ; Male (MeSH) ; MicroRNAs: metabolism (MeSH) ; Middle Aged (MeSH) ; Parkinson Disease: metabolism (MeSH) ; Parkinson Disease: physiopathology (MeSH) ; Severity of Illness Index (MeSH) ; Biomarker ; Gut biopsy ; Lewy body disease ; Parkinson's disease ; microRNA

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Contributing Institute(s):

1. Translational Neurodegeneration (AG Höglinger 1)
2. Clinical Research (Munich) (Clinical Research (Munich))

Research Program(s):

1. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2021

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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