Preprint

DZNE-2021-01038

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Revealing Spatiotemporal Circuit Information of Olfactory Bulb in Large-scale Neural Recordings

Hu, X. (First author)DZNE* ; Khanzada, S.DZNE* ; Klütsch, D.DZNE* ; Calegari, F. (Collaboration author) ; Amin, H. (Last author)DZNE*

2021

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Please use a persistent id in citations: doi:10.1101/2021.03.05.434081

Abstract: Large-scale multi-site biosensors are essential to probe the olfactory bulb (OB) circuitry for understanding the spatiotemporal dynamics of simultaneous discharge patterns. Current ex-vivo electrophysiological techniques are limited to recording a small set of neurons and cannot provide an inadequate resolution, which hinders revealing the fast dynamic underlying the information coding mechanisms in the OB circuit. Here, we demonstrate a novel biohybrid OB-CMOS platform to decipher the cross-scale dynamics of OB electrogenesis and quantify the distinct neuronal coding properties. The approach with 4096-microelectrodes offers a non-invasive, label-free, bioelectrical imaging to decode simultaneous firing patterns from thousands of connected neuronal ensembles in acute OB slices. The platform can measure spontaneous and drug-induced extracellular field potential activity. We employ our OB-CMOS recordings to perform multidimensional analysis to instantiate specific neurophysiological metrics underlying the olfactory spatiotemporal coding that emerged from the OB interconnected layers. Our results delineate the computational implications of large-scale activity patterns in functional olfactory processing. The high-content characterization of the olfactory circuit could benefit better functional interrogations of the olfactory spatiotemporal coding, connectivity mapping, and, further, the designing of reliable and advanced olfactory cell-based biosensors for diagnostic biomarkers and drug discovery.

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Contributing Institute(s):

1. Plasticity models in aging and neurodegeneration (AG Amin)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Hu, X. (First author)DZNE* ; Khanzada, S.DZNE* ; Klütsch, D.DZNE* ; Calegari, F. ; Amin, H. (Last author)DZNE*
Implementation of biohybrid olfactory bulb on a high-density CMOS-chip to reveal large-scale spatiotemporal circuit information.
Biosensors and bioelectronics 198, 113834 (2022) [10.1016/j.bios.2021.113834]2022   Files BibTeX | EndNote: XML, Text | RIS

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