TY  - JOUR
AU  - Graf, Jürgen
AU  - Zhang, Chuanqiang
AU  - Marguet, Stephan
AU  - Herrmann, Tanja
AU  - Flossmann, Tom
AU  - Hinsch, Robin
AU  - Rahmati, Vahid
AU  - Guenther, Madlen
AU  - Frahm, Christiane
AU  - Urbach, Anja
AU  - Neves, Ricardo Melo
AU  - Witte, Otto W
AU  - Kiebel, Stefan J
AU  - Isbrandt, Dirk
AU  - Hübner, Christian A
AU  - Holthoff, Knut
AU  - Kirmse, Knut
TI  - A limited role of NKCC1 in telencephalic glutamatergic neurons for developing hippocampal network dynamics and behavior.
JO  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 118
IS  - 14
SN  - 1091-6490
CY  - Washington, DC
PB  - National Acad. of Sciences
M1  - DZNE-2021-01223
SP  - e2014784118
PY  - 2021
AB  - NKCC1 is the primary transporter mediating chloride uptake in immature principal neurons, but its role in the development of in vivo network dynamics and cognitive abilities remains unknown. Here, we address the function of NKCC1 in developing mice using electrophysiological, optical, and behavioral approaches. We report that NKCC1 deletion from telencephalic glutamatergic neurons decreases in vitro excitatory actions of γ-aminobutyric acid (GABA) and impairs neuronal synchrony in neonatal hippocampal brain slices. In vivo, it has a minor impact on correlated spontaneous activity in the hippocampus and does not affect network activity in the intact visual cortex. Moreover, long-term effects of the developmental NKCC1 deletion on synaptic maturation, network dynamics, and behavioral performance are subtle. Our data reveal a neural network function of NKCC1 in hippocampal glutamatergic neurons in vivo, but challenge the hypothesis that NKCC1 is essential for major aspects of hippocampal development.
KW  - Animals
KW  - Animals, Newborn
KW  - Glutamic Acid: metabolism
KW  - Hippocampus: growth & development
KW  - Mice
KW  - Nerve Net
KW  - Neurons: metabolism
KW  - Solute Carrier Family 12, Member 2: physiology
KW  - Synapses: metabolism
KW  - Visual Cortex: physiology
KW  - gamma-Aminobutyric Acid: metabolism
KW  - GABA (Other)
KW  - NKCC1 (Other)
KW  - development (Other)
KW  - hippocampus (Other)
KW  - in vivo (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33782119
C2  - pmc:PMC8040628
DO  - DOI:10.1073/pnas.2014784118
UR  - https://pub.dzne.de/record/157766
ER  -