Journal Article

DZNE-2021-01223

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A limited role of NKCC1 in telencephalic glutamatergic neurons for developing hippocampal network dynamics and behavior.

Graf, J. ; Zhang, C. ; Marguet, S.DZNE* ; Herrmann, T. ; Flossmann, T. ; Hinsch, R.DZNE* ; Rahmati, V. ; Guenther, M. ; Frahm, C. ; Urbach, A. ; Neves, R. M.DZNE* ; Witte, O. W. ; Kiebel, S. J. ; Isbrandt, D.DZNE* ; Hübner, C. A. ; Holthoff, K. ; Kirmse, K.

2021
National Acad. of Sciences Washington, DC

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Please use a persistent id in citations: doi:10.1073/pnas.2014784118

Abstract: NKCC1 is the primary transporter mediating chloride uptake in immature principal neurons, but its role in the development of in vivo network dynamics and cognitive abilities remains unknown. Here, we address the function of NKCC1 in developing mice using electrophysiological, optical, and behavioral approaches. We report that NKCC1 deletion from telencephalic glutamatergic neurons decreases in vitro excitatory actions of γ-aminobutyric acid (GABA) and impairs neuronal synchrony in neonatal hippocampal brain slices. In vivo, it has a minor impact on correlated spontaneous activity in the hippocampus and does not affect network activity in the intact visual cortex. Moreover, long-term effects of the developmental NKCC1 deletion on synaptic maturation, network dynamics, and behavioral performance are subtle. Our data reveal a neural network function of NKCC1 in hippocampal glutamatergic neurons in vivo, but challenge the hypothesis that NKCC1 is essential for major aspects of hippocampal development.

Keyword(s): Animals (MeSH) ; Animals, Newborn (MeSH) ; Glutamic Acid: metabolism (MeSH) ; Hippocampus: growth & development (MeSH) ; Mice (MeSH) ; Nerve Net (MeSH) ; Neurons: metabolism (MeSH) ; Solute Carrier Family 12, Member 2: physiology (MeSH) ; Synapses: metabolism (MeSH) ; Visual Cortex: physiology (MeSH) ; gamma-Aminobutyric Acid: metabolism (MeSH) ; GABA ; NKCC1 ; development ; hippocampus ; in vivo

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Contributing Institute(s):

1. Experimental Neurophysiology (AG Isbrandt)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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