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@ARTICLE{Graf:157766,
author = {Graf, Jürgen and Zhang, Chuanqiang and Marguet, Stephan
and Herrmann, Tanja and Flossmann, Tom and Hinsch, Robin and
Rahmati, Vahid and Guenther, Madlen and Frahm, Christiane
and Urbach, Anja and Neves, Ricardo Melo and Witte, Otto W
and Kiebel, Stefan J and Isbrandt, Dirk and Hübner,
Christian A and Holthoff, Knut and Kirmse, Knut},
title = {{A} limited role of {NKCC}1 in telencephalic glutamatergic
neurons for developing hippocampal network dynamics and
behavior.},
journal = {Proceedings of the National Academy of Sciences of the
United States of America},
volume = {118},
number = {14},
issn = {1091-6490},
address = {Washington, DC},
publisher = {National Acad. of Sciences},
reportid = {DZNE-2021-01223},
pages = {e2014784118},
year = {2021},
abstract = {NKCC1 is the primary transporter mediating chloride uptake
in immature principal neurons, but its role in the
development of in vivo network dynamics and cognitive
abilities remains unknown. Here, we address the function of
NKCC1 in developing mice using electrophysiological,
optical, and behavioral approaches. We report that NKCC1
deletion from telencephalic glutamatergic neurons decreases
in vitro excitatory actions of γ-aminobutyric acid (GABA)
and impairs neuronal synchrony in neonatal hippocampal brain
slices. In vivo, it has a minor impact on correlated
spontaneous activity in the hippocampus and does not affect
network activity in the intact visual cortex. Moreover,
long-term effects of the developmental NKCC1 deletion on
synaptic maturation, network dynamics, and behavioral
performance are subtle. Our data reveal a neural network
function of NKCC1 in hippocampal glutamatergic neurons in
vivo, but challenge the hypothesis that NKCC1 is essential
for major aspects of hippocampal development.},
keywords = {Animals / Animals, Newborn / Glutamic Acid: metabolism /
Hippocampus: growth $\&$ development / Mice / Nerve Net /
Neurons: metabolism / Solute Carrier Family 12, Member 2:
physiology / Synapses: metabolism / Visual Cortex:
physiology / gamma-Aminobutyric Acid: metabolism / GABA
(Other) / NKCC1 (Other) / development (Other) / hippocampus
(Other) / in vivo (Other)},
cin = {AG Isbrandt},
ddc = {500},
cid = {I:(DE-2719)1011003},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33782119},
pmc = {pmc:PMC8040628},
doi = {10.1073/pnas.2014784118},
url = {https://pub.dzne.de/record/157766},
}