%0 Journal Article
%A Schinke, Christian
%A Fernandez Vallone, Valeria
%A Ivanov, Andranik
%A Peng, Yangfan
%A Körtvelyessy, Péter
%A Nolte, Luca
%A Huehnchen, Petra
%A Beule, Dieter
%A Stachelscheid, Harald
%A Boehmerle, Wolfgang
%A Endres, Matthias
%T Dataset for: Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons.
%J Data in Brief
%V 38
%@ 2352-3409
%C Amsterdam [u.a.]
%I Elsevier
%M DZNE-2021-01501
%P 107320
%D 2021
%Z (CC BY-NC-ND)
%X Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and potentially irreversible adverse event of cytotoxic chemotherapy. We evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for chemotherapy induced neurotoxicity. Sensory neurons differentiated from two established induced pluripotent stem cell lines were used (s.c. BIHi005-A https://hpscreg.eu/cell-line/BIHi005-A and BIHi004-B https://hpscreg.eu/cell-line/BIHi004-B, Berlin Institute of Health Stem Cell Core Facility). Cell viability and cytotoxicity assays were performed, comparing susceptibility to four neurotoxic and two non-neurotoxic drugs. RNA sequencing analyses in paclitaxel vs. vehicle (DMSO)-treated sensory neurons were performed. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to a dose dependent decline of cell viability in clinically relevant IC50 ranges, which was not the case for the non-neurotoxic compounds doxorubicin and 5-fluorouracil. RNA sequencing analyses at 24 h, i.e. before paclitaxel-induced cell death occurred, revealed the differential expression of genes of neuronal injury, cellular stress response, and sterol pathways in response to 1 µM paclitaxel. Neuroprotective effects of lithium chloride co-incubation, which were previously shown in rodent dorsal root ganglia, could be replicated in human iPSC-DSN. Cell lines from the two different donors BIHi005-A and BIHi004-B showed different responses to the neurotoxic treatment in cell viability and cytotoxicity assays.
%K 3R (Other)
%K Chemotherapy induced neuropathy (Other)
%K Induced pluripotent stem cell derived sensory neurons (iPSC-DSN) (Other)
%K Lithium (Other)
%K Replacement (Other)
%K Transcriptome (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34485650
%2 pmc:PMC8408513
%R 10.1016/j.dib.2021.107320
%U https://pub.dzne.de/record/162846