Journal Article

DZNE-2021-01501

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset for: Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons.

Schinke, C. ; Fernandez Vallone, V. ; Ivanov, A. ; Peng, Y. ; Körtvelyessy, P.DZNE* ; Nolte, L. ; Huehnchen, P. ; Beule, D. ; Stachelscheid, H. ; Boehmerle, W. ; Endres, M. (Last author)DZNE*

2021
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.dib.2021.107320

Abstract: Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and potentially irreversible adverse event of cytotoxic chemotherapy. We evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for chemotherapy induced neurotoxicity. Sensory neurons differentiated from two established induced pluripotent stem cell lines were used (s.c. BIHi005-A https://hpscreg.eu/cell-line/BIHi005-A and BIHi004-B https://hpscreg.eu/cell-line/BIHi004-B, Berlin Institute of Health Stem Cell Core Facility). Cell viability and cytotoxicity assays were performed, comparing susceptibility to four neurotoxic and two non-neurotoxic drugs. RNA sequencing analyses in paclitaxel vs. vehicle (DMSO)-treated sensory neurons were performed. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to a dose dependent decline of cell viability in clinically relevant IC50 ranges, which was not the case for the non-neurotoxic compounds doxorubicin and 5-fluorouracil. RNA sequencing analyses at 24 h, i.e. before paclitaxel-induced cell death occurred, revealed the differential expression of genes of neuronal injury, cellular stress response, and sterol pathways in response to 1 µM paclitaxel. Neuroprotective effects of lithium chloride co-incubation, which were previously shown in rodent dorsal root ganglia, could be replicated in human iPSC-DSN. Cell lines from the two different donors BIHi005-A and BIHi004-B showed different responses to the neurotoxic treatment in cell viability and cytotoxicity assays.

Keyword(s): 3R ; Chemotherapy induced neuropathy ; Induced pluripotent stem cell derived sensory neurons (iPSC-DSN) ; Lithium ; Replacement ; Transcriptome

Note: (CC BY-NC-ND)

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Contributing Institute(s):

1. Interdisciplinary Dementia Research (AG Endres)
2. Clinical Neurophysiology and Memory (AG Düzel)

Research Program(s):

1. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2021

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; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Schinke, C. ; Fernandez Vallone, V. ; Ivanov, A. ; Peng, Y. ; Körtvelyessy, P.DZNE* ; Nolte, L. ; Huehnchen, P. ; Beule, D. ; Stachelscheid, H. ; Boehmerle, W. ; Endres, M. (Last author)DZNE*
Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons.
Neurobiology of disease 155, 105391 (2021) [10.1016/j.nbd.2021.105391]2021   Files BibTeX | EndNote: XML, Text | RIS

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