TY  - JOUR
AU  - Schinke, Christian
AU  - Fernandez Vallone, Valeria
AU  - Ivanov, Andranik
AU  - Peng, Yangfan
AU  - Körtvelyessy, Péter
AU  - Nolte, Luca
AU  - Huehnchen, Petra
AU  - Beule, Dieter
AU  - Stachelscheid, Harald
AU  - Boehmerle, Wolfgang
AU  - Endres, Matthias
TI  - Dataset for: Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC)-derived sensory neurons.
JO  - Data in Brief
VL  - 38
SN  - 2352-3409
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - DZNE-2021-01501
SP  - 107320
PY  - 2021
N1  - (CC BY-NC-ND)
AB  - Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent and potentially irreversible adverse event of cytotoxic chemotherapy. We evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for chemotherapy induced neurotoxicity. Sensory neurons differentiated from two established induced pluripotent stem cell lines were used (s.c. BIHi005-A https://hpscreg.eu/cell-line/BIHi005-A and BIHi004-B https://hpscreg.eu/cell-line/BIHi004-B, Berlin Institute of Health Stem Cell Core Facility). Cell viability and cytotoxicity assays were performed, comparing susceptibility to four neurotoxic and two non-neurotoxic drugs. RNA sequencing analyses in paclitaxel vs. vehicle (DMSO)-treated sensory neurons were performed. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to a dose dependent decline of cell viability in clinically relevant IC50 ranges, which was not the case for the non-neurotoxic compounds doxorubicin and 5-fluorouracil. RNA sequencing analyses at 24 h, i.e. before paclitaxel-induced cell death occurred, revealed the differential expression of genes of neuronal injury, cellular stress response, and sterol pathways in response to 1 µM paclitaxel. Neuroprotective effects of lithium chloride co-incubation, which were previously shown in rodent dorsal root ganglia, could be replicated in human iPSC-DSN. Cell lines from the two different donors BIHi005-A and BIHi004-B showed different responses to the neurotoxic treatment in cell viability and cytotoxicity assays.
KW  - 3R (Other)
KW  - Chemotherapy induced neuropathy (Other)
KW  - Induced pluripotent stem cell derived sensory neurons (iPSC-DSN) (Other)
KW  - Lithium (Other)
KW  - Replacement (Other)
KW  - Transcriptome (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:34485650
C2  - pmc:PMC8408513
DO  - DOI:10.1016/j.dib.2021.107320
UR  - https://pub.dzne.de/record/162846
ER  -