TY  - JOUR
AU  - Wieghofer, Peter
AU  - Hagemeyer, Nora
AU  - Sankowski, Roman
AU  - Schlecht, Anja
AU  - Staszewski, Ori
AU  - Amann, Lukas
AU  - Gruber, Markus
AU  - Koch, Jana
AU  - Hausmann, Annika
AU  - Zhang, Peipei
AU  - Boneva, Stefaniya
AU  - Masuda, Takahiro
AU  - Hilgendorf, Ingo
AU  - Goldmann, Tobias
AU  - Böttcher, Chotima
AU  - Priller, Josef
AU  - Rossi, Fabio Mv
AU  - Lange, Clemens
AU  - Prinz, Marco
TI  - Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.
JO  - The EMBO journal
VL  - 40
IS  - 6
SN  - 0261-4189
CY  - Hoboken, NJ [u.a.]
PB  - Wiley
M1  - DZNE-2022-00856
SP  - e105123
PY  - 2021
AB  - Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.
KW  - Animals
KW  - Choroid: blood supply
KW  - Choroid: embryology
KW  - Computational Biology
KW  - Computer Simulation
KW  - Eye: cytology
KW  - Eye: immunology
KW  - Eye: metabolism
KW  - Female
KW  - Homeostasis: immunology
KW  - Humans
KW  - Immunity, Innate: immunology
KW  - Macrophages: immunology
KW  - Macular Degeneration: pathology
KW  - Male
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Mice, Transgenic
KW  - Microglia: physiology
KW  - Myeloid Cells: immunology
KW  - Myeloid Cells: metabolism
KW  - Neovascularization, Physiologic: physiology
KW  - Sequence Analysis, RNA
KW  - Single-Cell Analysis
KW  - Transcription, Genetic: genetics
KW  - cornea (Other)
KW  - macrophages (Other)
KW  - microglia (Other)
KW  - retina (Other)
KW  - single-cell RNA-seq (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33555074
C2  - pmc:PMC7957431
DO  - DOI:10.15252/embj.2020105123
UR  - https://pub.dzne.de/record/164200
ER  -