Journal Article

DZNE-2022-00856

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

Wieghofer, P.Extern* ; Hagemeyer, N. ; Sankowski, R. ; Schlecht, A. ; Staszewski, O. ; Amann, L. ; Gruber, M. ; Koch, J. ; Hausmann, A. ; Zhang, P. ; Boneva, S. ; Masuda, T. ; Hilgendorf, I. ; Goldmann, T. ; Böttcher, C.Extern* ; Priller, J.DZNE* ; Rossi, F. M. ; Lange, C. ; Prinz, M.

2021
Wiley Hoboken, NJ [u.a.]

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Please use a persistent id in citations: doi:10.15252/embj.2020105123

Abstract: Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.

Keyword(s): Animals (MeSH) ; Choroid: blood supply (MeSH) ; Choroid: embryology (MeSH) ; Computational Biology (MeSH) ; Computer Simulation (MeSH) ; Eye: cytology (MeSH) ; Eye: immunology (MeSH) ; Eye: metabolism (MeSH) ; Female (MeSH) ; Homeostasis: immunology (MeSH) ; Humans (MeSH) ; Immunity, Innate: immunology (MeSH) ; Macrophages: immunology (MeSH) ; Macular Degeneration: pathology (MeSH) ; Male (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Mice, Transgenic (MeSH) ; Microglia: physiology (MeSH) ; Myeloid Cells: immunology (MeSH) ; Myeloid Cells: metabolism (MeSH) ; Neovascularization, Physiologic: physiology (MeSH) ; Sequence Analysis, RNA (MeSH) ; Single-Cell Analysis (MeSH) ; Transcription, Genetic: genetics (MeSH) ; cornea ; macrophages ; microglia ; retina ; single-cell RNA-seq

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Contributing Institute(s):

1. Translational Neuropsychiatry (AG Priller)

Research Program(s):

1. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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