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@ARTICLE{Wieghofer:164200,
author = {Wieghofer, Peter and Hagemeyer, Nora and Sankowski, Roman
and Schlecht, Anja and Staszewski, Ori and Amann, Lukas and
Gruber, Markus and Koch, Jana and Hausmann, Annika and
Zhang, Peipei and Boneva, Stefaniya and Masuda, Takahiro and
Hilgendorf, Ingo and Goldmann, Tobias and Böttcher, Chotima
and Priller, Josef and Rossi, Fabio Mv and Lange, Clemens
and Prinz, Marco},
title = {{M}apping the origin and fate of myeloid cells in distinct
compartments of the eye by single-cell profiling.},
journal = {The EMBO journal},
volume = {40},
number = {6},
issn = {0261-4189},
address = {Hoboken, NJ [u.a.]},
publisher = {Wiley},
reportid = {DZNE-2022-00856},
pages = {e105123},
year = {2021},
abstract = {Similar to the brain, the eye is considered an
immune-privileged organ where tissue-resident macrophages
provide the major immune cell constituents. However, little
is known about spatially restricted macrophage subsets
within different eye compartments with regard to their
origin, function, and fate during health and disease. Here,
we combined single-cell analysis, fate mapping, parabiosis,
and computational modeling to comprehensively examine
myeloid subsets in distinct parts of the eye during
homeostasis. This approach allowed us to identify myeloid
subsets displaying diverse transcriptional states. During
choroidal neovascularization, a typical hallmark of
neovascular age-related macular degeneration (AMD), we
recognized disease-specific macrophage subpopulations with
distinct molecular signatures. Our results highlight the
heterogeneity of myeloid subsets and their dynamics in the
eye that provide new insights into the innate immune system
in this organ which may offer new therapeutic targets for
ophthalmological diseases.},
keywords = {Animals / Choroid: blood supply / Choroid: embryology /
Computational Biology / Computer Simulation / Eye: cytology
/ Eye: immunology / Eye: metabolism / Female / Homeostasis:
immunology / Humans / Immunity, Innate: immunology /
Macrophages: immunology / Macular Degeneration: pathology /
Male / Mice / Mice, Inbred C57BL / Mice, Transgenic /
Microglia: physiology / Myeloid Cells: immunology / Myeloid
Cells: metabolism / Neovascularization, Physiologic:
physiology / Sequence Analysis, RNA / Single-Cell Analysis /
Transcription, Genetic: genetics / cornea (Other) /
macrophages (Other) / microglia (Other) / retina (Other) /
single-cell RNA-seq (Other)},
cin = {AG Priller},
ddc = {570},
cid = {I:(DE-2719)5000007},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33555074},
pmc = {pmc:PMC7957431},
doi = {10.15252/embj.2020105123},
url = {https://pub.dzne.de/record/164200},
}