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@ARTICLE{Mieskes:164441,
      author       = {Mieskes, Frank and Wehnekamp, Fabian and Plucińska,
                      Gabriela and Thong, Rachel and Misgeld, Thomas and Lamb, Don
                      C},
      title        = {{T}rajectory data of antero- and retrograde movement of
                      mitochondria in living zebrafish larvae.},
      journal      = {Data in Brief},
      volume       = {29},
      issn         = {2352-3409},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DZNE-2022-00993},
      pages        = {105280},
      year         = {2020},
      note         = {(CC BY 4.0)},
      abstract     = {Recently, a large number of single particle tracking (SPT)
                      approaches have been developed. Generally, SPT techniques
                      can be split into two groups: ex post facto approaches where
                      trajectory extraction is carried out after data acquisition
                      and feedback based approaches that perform particle tracking
                      in real time [1]. One feedback approach is 3D Orbital
                      Tracking, where the laser excitation beam is rotated in a
                      circle about the object, generating a so called orbit [2,3].
                      By calculating the particle position from the detected
                      intensity after every orbit in relation to its center, this
                      method allows the microscope to follow a single object in
                      real time. The high spatiotemporal resolution of this method
                      and the potential to optically manipulate the followed
                      object during the measurement promises to yield new deep
                      insights into biological systems [4-7]. By upgrading this
                      approach in a way that the specimen is recentered by a
                      xy-stage on the center of the microscope, particle tracking
                      with this long-range tracking feature is no longer limited
                      to the covered field-of-view. This allows for the
                      observation of mitochondrial trafficking in living zebrafish
                      embryos over long distances. Here, we provide the raw data
                      for antero- and retrograde movement of mitochondria labelled
                      with photo-activatable green fluorescent protein
                      (mitoPAGFP). It relates to the scientific article
                      'Nanoresolution real-time 3D orbital tracking for studying
                      mitochondrial trafficking in vertebrate axons in vivo' [8].
                      By applying a correlation analysis on the trajectories, it
                      is possible to distinguish between active transport and
                      pausing events with less biasing compared to the mean
                      squared displacement approach.},
      keywords     = {Fluorescence (Other) / Mitochondria trafficking (Other) /
                      Orbital tracking (Other) / Single particle tracking (Other)
                      / Transport (Other)},
      cin          = {AG Misgeld / München common},
      ddc          = {570},
      cid          = {I:(DE-2719)1110000-4 / I:(DE-2719)6000016},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32190718},
      pmc          = {pmc:PMC7068625},
      doi          = {10.1016/j.dib.2020.105280},
      url          = {https://pub.dzne.de/record/164441},
}