Journal Article

DZNE-2022-00993

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Trajectory data of antero- and retrograde movement of mitochondria in living zebrafish larvae.

Mieskes, F. ; Wehnekamp, F. ; Plucińska, G.DZNE* ; Thong, R.DZNE* ; Misgeld, T.DZNE* ; Lamb, D. C.

2020
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.dib.2020.105280

Abstract: Recently, a large number of single particle tracking (SPT) approaches have been developed. Generally, SPT techniques can be split into two groups: ex post facto approaches where trajectory extraction is carried out after data acquisition and feedback based approaches that perform particle tracking in real time [1]. One feedback approach is 3D Orbital Tracking, where the laser excitation beam is rotated in a circle about the object, generating a so called orbit [2,3]. By calculating the particle position from the detected intensity after every orbit in relation to its center, this method allows the microscope to follow a single object in real time. The high spatiotemporal resolution of this method and the potential to optically manipulate the followed object during the measurement promises to yield new deep insights into biological systems [4-7]. By upgrading this approach in a way that the specimen is recentered by a xy-stage on the center of the microscope, particle tracking with this long-range tracking feature is no longer limited to the covered field-of-view. This allows for the observation of mitochondrial trafficking in living zebrafish embryos over long distances. Here, we provide the raw data for antero- and retrograde movement of mitochondria labelled with photo-activatable green fluorescent protein (mitoPAGFP). It relates to the scientific article 'Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo' [8]. By applying a correlation analysis on the trajectories, it is possible to distinguish between active transport and pausing events with less biasing compared to the mean squared displacement approach.

Keyword(s): Fluorescence ; Mitochondria trafficking ; Orbital tracking ; Single particle tracking ; Transport

Note: (CC BY 4.0)

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Contributing Institute(s):

1. Neuronal Cell Biology (AG Misgeld)
2. München common (München common)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2020

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Database coverage:
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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Wehnekamp, F. ; Plucińska, G.DZNE* ; Thong, R.DZNE* ; Misgeld, T. (Corresponding author)DZNE* ; Lamb, D. C.
Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo.
eLife 8, e46059 (2019) [10.7554/eLife.46059]2019   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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