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000164600 020__ $$a978-1-4939-2549-0 (print)
000164600 020__ $$a978-1-4939-2550-6 (electronic)
000164600 0247_ $$2pmid$$apmid:25820711
000164600 0247_ $$2doi$$a10.1007/978-1-4939-2550-6_3
000164600 0247_ $$2ISSN$$a1064-3745
000164600 0247_ $$2ISSN$$a1940-6029
000164600 037__ $$aDZNE-2022-01143
000164600 082__ $$a570
000164600 1001_ $$aPosch, Anton$$b0$$eEditor
000164600 245__ $$aIsolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting
000164600 260__ $$aNew York, NY$$bSpringer New York$$c2015
000164600 29510 $$aProteomic Profiling / Posch, Anton (Editor) ; New York, NY : Springer New York, 2015, Chapter 3 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-2549-0=978-1-4939-2550-6 ; doi:10.1007/978-1-4939-2550-6
000164600 300__ $$a33 - 42
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000164600 3367_ $$2BibTeX$$aINBOOK
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000164600 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1654677993_2868
000164600 4900_ $$aMethods in Molecular Biology$$v1295
000164600 520__ $$aCombining density gradient centrifugation with magnetic cell sorting provides a powerful tool to isolate blood cells with high reproducibility, yield, and purity. It also allows for subsequent separation of multiple cell types, resulting in the possibility to analyze different purified fractions from one donor’s sample. The centrifugation step divides whole blood into peripheral blood mononuclear cells (PBMC), erythrocytes, and platelet-rich plasma. In the following, lymphocyte subtypes can be consecutively isolated from the PBMC fraction. This chapter describes enrichment of erythrocytes, CD14-positive monocytes and CD3-positive T lymphocytes. Alternatively, other cell types can be targeted by using magnetic beads specific for the desired subpopulation.
000164600 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0
000164600 588__ $$aDataset connected to CrossRef Book Series, Journals: pub.dzne.de
000164600 650_2 $$2MeSH$$aAntigens, CD: metabolism
000164600 650_2 $$2MeSH$$aCentrifugation, Density Gradient: methods
000164600 650_2 $$2MeSH$$aFlow Cytometry: methods
000164600 650_2 $$2MeSH$$aHumans
000164600 650_2 $$2MeSH$$aImmunomagnetic Separation: methods
000164600 650_2 $$2MeSH$$aImmunophenotyping
000164600 650_2 $$2MeSH$$aLeukocytes, Mononuclear: cytology
000164600 650_2 $$2MeSH$$aLeukocytes, Mononuclear: metabolism
000164600 650_2 $$2MeSH$$aLymphocytes: cytology
000164600 650_2 $$2MeSH$$aLymphocytes: metabolism
000164600 7001_ $$0P:(DE-2719)2810593$$aBrosseron, Frederic$$b1$$eFirst author$$udzne
000164600 7001_ $$aMarcus, Katrin$$b2
000164600 7001_ $$aMay, Caroline$$b3
000164600 773__ $$a10.1007/978-1-4939-2550-6_3
000164600 909CO $$ooai:pub.dzne.de:164600$$pVDB
000164600 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810593$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
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000164600 9141_ $$y2015
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000164600 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-09-11
000164600 9201_ $$0I:(DE-2719)1011301$$kAG Heneka1 ; AG Heneka 1$$lInterventional Trials and Biomarkers in Neurodegenerative Diseases$$x0
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