Contribution to a book

DZNE-2022-01143

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting

Posch, A. (Editor) ; Brosseron, F. (First author)DZNE* ; Marcus, K. ; May, C.

2015
Springer New York New York, NY
ISBN: 978-1-4939-2549-0 (print), 978-1-4939-2550-6 (electronic)

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Please use a persistent id in citations: doi:10.1007/978-1-4939-2550-6_3

Abstract: Combining density gradient centrifugation with magnetic cell sorting provides a powerful tool to isolate blood cells with high reproducibility, yield, and purity. It also allows for subsequent separation of multiple cell types, resulting in the possibility to analyze different purified fractions from one donor’s sample. The centrifugation step divides whole blood into peripheral blood mononuclear cells (PBMC), erythrocytes, and platelet-rich plasma. In the following, lymphocyte subtypes can be consecutively isolated from the PBMC fraction. This chapter describes enrichment of erythrocytes, CD14-positive monocytes and CD3-positive T lymphocytes. Alternatively, other cell types can be targeted by using magnetic beads specific for the desired subpopulation.

Keyword(s): Antigens, CD: metabolism (MeSH) ; Centrifugation, Density Gradient: methods (MeSH) ; Flow Cytometry: methods (MeSH) ; Humans (MeSH) ; Immunomagnetic Separation: methods (MeSH) ; Immunophenotyping (MeSH) ; Leukocytes, Mononuclear: cytology (MeSH) ; Leukocytes, Mononuclear: metabolism (MeSH) ; Lymphocytes: cytology (MeSH) ; Lymphocytes: metabolism (MeSH)

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Contributing Institute(s):

1. Interventional Trials and Biomarkers in Neurodegenerative Diseases (AG Heneka1 ; AG Heneka 1)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2015

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Database coverage:
; SCOPUS

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