TY  - CHAP
AU  - Brosseron, Frederic
AU  - Marcus, Katrin
AU  - May, Caroline
A3  - Posch, Anton
TI  - Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting
VL  - 1295
CY  - New York, NY
PB  - Springer New York
M1  - DZNE-2022-01143
SN  - 978-1-4939-2549-0 (print)
T2  - Methods in Molecular Biology
SP  - 33 - 42
PY  - 2015
AB  - Combining density gradient centrifugation with magnetic cell sorting provides a powerful tool to isolate blood cells with high reproducibility, yield, and purity. It also allows for subsequent separation of multiple cell types, resulting in the possibility to analyze different purified fractions from one donor’s sample. The centrifugation step divides whole blood into peripheral blood mononuclear cells (PBMC), erythrocytes, and platelet-rich plasma. In the following, lymphocyte subtypes can be consecutively isolated from the PBMC fraction. This chapter describes enrichment of erythrocytes, CD14-positive monocytes and CD3-positive T lymphocytes. Alternatively, other cell types can be targeted by using magnetic beads specific for the desired subpopulation.
KW  - Antigens, CD: metabolism
KW  - Centrifugation, Density Gradient: methods
KW  - Flow Cytometry: methods
KW  - Humans
KW  - Immunomagnetic Separation: methods
KW  - Immunophenotyping
KW  - Leukocytes, Mononuclear: cytology
KW  - Leukocytes, Mononuclear: metabolism
KW  - Lymphocytes: cytology
KW  - Lymphocytes: metabolism
LB  - PUB:(DE-HGF)7
C6  - pmid:25820711
DO  - DOI:10.1007/978-1-4939-2550-6_3
UR  - https://pub.dzne.de/record/164600
ER  -