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@INBOOK{Posch:164600,
      author       = {Brosseron, Frederic and Marcus, Katrin and May, Caroline},
      editor       = {Posch, Anton},
      title        = {{I}solating {P}eripheral {L}ymphocytes by {D}ensity
                      {G}radient {C}entrifugation and {M}agnetic {C}ell {S}orting},
      volume       = {1295},
      address      = {New York, NY},
      publisher    = {Springer New York},
      reportid     = {DZNE-2022-01143},
      isbn         = {978-1-4939-2549-0 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {33 - 42},
      year         = {2015},
      comment      = {Proteomic Profiling / Posch, Anton (Editor) ; New York, NY
                      : Springer New York, 2015, Chapter 3 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-4939-2549-0=978-1-4939-2550-6 ;
                      doi:10.1007/978-1-4939-2550-6},
      booktitle     = {Proteomic Profiling / Posch, Anton
                       (Editor) ; New York, NY : Springer New
                       York, 2015, Chapter 3 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-4939-2549-0=978-1-4939-2550-6 ;
                       doi:10.1007/978-1-4939-2550-6},
      abstract     = {Combining density gradient centrifugation with magnetic
                      cell sorting provides a powerful tool to isolate blood cells
                      with high reproducibility, yield, and purity. It also allows
                      for subsequent separation of multiple cell types, resulting
                      in the possibility to analyze different purified fractions
                      from one donor’s sample. The centrifugation step divides
                      whole blood into peripheral blood mononuclear cells (PBMC),
                      erythrocytes, and platelet-rich plasma. In the following,
                      lymphocyte subtypes can be consecutively isolated from the
                      PBMC fraction. This chapter describes enrichment of
                      erythrocytes, CD14-positive monocytes and CD3-positive T
                      lymphocytes. Alternatively, other cell types can be targeted
                      by using magnetic beads specific for the desired
                      subpopulation.},
      keywords     = {Antigens, CD: metabolism / Centrifugation, Density
                      Gradient: methods / Flow Cytometry: methods / Humans /
                      Immunomagnetic Separation: methods / Immunophenotyping /
                      Leukocytes, Mononuclear: cytology / Leukocytes, Mononuclear:
                      metabolism / Lymphocytes: cytology / Lymphocytes:
                      metabolism},
      cin          = {AG Heneka1 ; AG Heneka 1},
      ddc          = {570},
      cid          = {I:(DE-2719)1011301},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:25820711},
      doi          = {10.1007/978-1-4939-2550-6_3},
      url          = {https://pub.dzne.de/record/164600},
}