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001     164600
005     20230425135644.0
020 _ _ |a 978-1-4939-2549-0 (print)
020 _ _ |a 978-1-4939-2550-6 (electronic)
024 7 _ |a pmid:25820711
|2 pmid
024 7 _ |a 10.1007/978-1-4939-2550-6_3
|2 doi
024 7 _ |a 1064-3745
|2 ISSN
024 7 _ |a 1940-6029
|2 ISSN
037 _ _ |a DZNE-2022-01143
082 _ _ |a 570
100 1 _ |a Posch, Anton
|b 0
|e Editor
245 _ _ |a Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting
260 _ _ |a New York, NY
|c 2015
|b Springer New York
295 1 0 |a Proteomic Profiling / Posch, Anton (Editor) ; New York, NY : Springer New York, 2015, Chapter 3 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-2549-0=978-1-4939-2550-6 ; doi:10.1007/978-1-4939-2550-6
300 _ _ |a 33 - 42
336 7 _ |a BOOK_CHAPTER
|2 ORCID
336 7 _ |a Book Section
|0 7
|2 EndNote
336 7 _ |a bookPart
|2 DRIVER
336 7 _ |a INBOOK
|2 BibTeX
336 7 _ |a Output Types/Book chapter
|2 DataCite
336 7 _ |a Contribution to a book
|b contb
|m contb
|0 PUB:(DE-HGF)7
|s 1654677993_2868
|2 PUB:(DE-HGF)
490 0 _ |a Methods in Molecular Biology
|v 1295
520 _ _ |a Combining density gradient centrifugation with magnetic cell sorting provides a powerful tool to isolate blood cells with high reproducibility, yield, and purity. It also allows for subsequent separation of multiple cell types, resulting in the possibility to analyze different purified fractions from one donor’s sample. The centrifugation step divides whole blood into peripheral blood mononuclear cells (PBMC), erythrocytes, and platelet-rich plasma. In the following, lymphocyte subtypes can be consecutively isolated from the PBMC fraction. This chapter describes enrichment of erythrocytes, CD14-positive monocytes and CD3-positive T lymphocytes. Alternatively, other cell types can be targeted by using magnetic beads specific for the desired subpopulation.
536 _ _ |a 899 - ohne Topic (POF4-899)
|0 G:(DE-HGF)POF4-899
|c POF4-899
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef Book Series, Journals: pub.dzne.de
650 _ 2 |a Antigens, CD: metabolism
|2 MeSH
650 _ 2 |a Centrifugation, Density Gradient: methods
|2 MeSH
650 _ 2 |a Flow Cytometry: methods
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Immunomagnetic Separation: methods
|2 MeSH
650 _ 2 |a Immunophenotyping
|2 MeSH
650 _ 2 |a Leukocytes, Mononuclear: cytology
|2 MeSH
650 _ 2 |a Leukocytes, Mononuclear: metabolism
|2 MeSH
650 _ 2 |a Lymphocytes: cytology
|2 MeSH
650 _ 2 |a Lymphocytes: metabolism
|2 MeSH
700 1 _ |a Brosseron, Frederic
|0 P:(DE-2719)2810593
|b 1
|e First author
|u dzne
700 1 _ |a Marcus, Katrin
|b 2
700 1 _ |a May, Caroline
|b 3
773 _ _ |a 10.1007/978-1-4939-2550-6_3
909 C O |o oai:pub.dzne.de:164600
|p VDB
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 1
|6 P:(DE-2719)2810593
913 1 _ |a DE-HGF
|b Programmungebundene Forschung
|l ohne Programm
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914 1 _ |y 2015
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
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|d 2020-09-11
915 _ _ |a DBCoverage
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|b Medline
|d 2020-09-11
920 1 _ |0 I:(DE-2719)1011301
|k AG Heneka1 ; AG Heneka 1
|l Interventional Trials and Biomarkers in Neurodegenerative Diseases
|x 0
980 _ _ |a contb
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1011301
980 _ _ |a UNRESTRICTED

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