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000164601 020__ $$a978-1-62703-522-4 (print)
000164601 020__ $$a978-1-62703-523-1 (electronic)
000164601 0247_ $$2doi$$a10.1007/978-1-62703-523-1_8
000164601 0247_ $$2ISSN$$a1064-3745
000164601 0247_ $$2ISSN$$a1940-6029
000164601 0247_ $$2altmetric$$aaltmetric:22261264
000164601 0247_ $$2pmid$$apmid:23852599
000164601 037__ $$aDZNE-2022-01144
000164601 082__ $$a570
000164601 1001_ $$aDe Nardo, Christine M.$$b0$$eEditor
000164601 245__ $$aASC Speck Formation as a Readout for Inflammasome Activation
000164601 260__ $$aTotowa, NJ$$bHumana Press$$c2013
000164601 29510 $$aThe Inflammasome / De Nardo, Christine M. (Editor) ; Totowa, NJ : Humana Press, 2013, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-62703-522-4=978-1-62703-523-1 ; doi:10.1007/978-1-62703-523-1
000164601 300__ $$a91 - 101
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000164601 3367_ $$2BibTeX$$aINBOOK
000164601 3367_ $$2DataCite$$aOutput Types/Book chapter
000164601 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1654678582_2870
000164601 4900_ $$aMethods in Molecular Biology$$v1040
000164601 520__ $$aAll inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed 'speck'. ASC specks can be observed as they reach a size of around 1 μm and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC speck visualization. First, we describe the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC. We then discuss stimulation conditions and the microscopic evaluation of ASC speck formation.
000164601 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0
000164601 588__ $$aDataset connected to CrossRef Book Series, Journals: pub.dzne.de
000164601 7001_ $$aLatz, Eicke$$b1$$eEditor
000164601 7001_ $$aStutz, Andrea$$b2
000164601 7001_ $$aHorvath, Gabor L.$$b3
000164601 7001_ $$aMonks, Brian G.$$b4
000164601 7001_ $$0P:(DE-2719)2000062$$aLatz, Eicke$$b5$$eLast author$$udzne
000164601 773__ $$a10.1007/978-1-62703-523-1_8
000164601 909CO $$ooai:pub.dzne.de:164601$$pVDB
000164601 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000062$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b5$$kDZNE
000164601 9131_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000164601 9141_ $$y2013
000164601 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-09-11
000164601 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-09-11
000164601 9201_ $$0I:(DE-2719)1013024$$kAG Latz$$lInnate Immunity in Neurodegeneration$$x0
000164601 980__ $$acontb
000164601 980__ $$aVDB
000164601 980__ $$aI:(DE-2719)1013024
000164601 980__ $$aUNRESTRICTED