Contribution to a book

DZNE-2022-01144

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png ASC Speck Formation as a Readout for Inflammasome Activation

De Nardo, C. M. (Editor) ; Latz, E. (Editor) ; Stutz, A. ; Horvath, G. L. ; Monks, B. G. ; Latz, E. (Last author)DZNE*

2013
Humana Press Totowa, NJ
ISBN: 978-1-62703-522-4 (print), 978-1-62703-523-1 (electronic)

This record in other databases:    

Please use a persistent id in citations: doi:10.1007/978-1-62703-523-1_8

Abstract: All inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed 'speck'. ASC specks can be observed as they reach a size of around 1 μm and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC speck visualization. First, we describe the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC. We then discuss stimulation conditions and the microscopic evaluation of ASC speck formation.

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Contributing Institute(s):

1. Innate Immunity in Neurodegeneration (AG Latz)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2013

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Database coverage:
; SCOPUS

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