TY  - CHAP
AU  - Stutz, Andrea
AU  - Horvath, Gabor L.
AU  - Monks, Brian G.
AU  - Latz, Eicke
A3  - De Nardo, Christine M.
A3  - Latz, Eicke
TI  - ASC Speck Formation as a Readout for Inflammasome Activation
VL  - 1040
CY  - Totowa, NJ
PB  - Humana Press
M1  - DZNE-2022-01144
SN  - 978-1-62703-522-4 (print)
T2  - Methods in Molecular Biology
SP  - 91 - 101
PY  - 2013
AB  - All inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed 'speck'. ASC specks can be observed as they reach a size of around 1 μm and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC speck visualization. First, we describe the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC. We then discuss stimulation conditions and the microscopic evaluation of ASC speck formation.
LB  - PUB:(DE-HGF)7
C6  - pmid:23852599
DO  - DOI:10.1007/978-1-62703-523-1_8
UR  - https://pub.dzne.de/record/164601
ER  -