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@INBOOK{DeNardo:164601,
      author       = {Stutz, Andrea and Horvath, Gabor L. and Monks, Brian G. and
                      Latz, Eicke},
      editor       = {De Nardo, Christine M. and Latz, Eicke},
      title        = {{ASC} {S}peck {F}ormation as a {R}eadout for {I}nflammasome
                      {A}ctivation},
      volume       = {1040},
      address      = {Totowa, NJ},
      publisher    = {Humana Press},
      reportid     = {DZNE-2022-01144},
      isbn         = {978-1-62703-522-4 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {91 - 101},
      year         = {2013},
      comment      = {The Inflammasome / De Nardo, Christine M. (Editor) ;
                      Totowa, NJ : Humana Press, 2013, Chapter 8 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-62703-522-4=978-1-62703-523-1 ;
                      doi:10.1007/978-1-62703-523-1},
      booktitle     = {The Inflammasome / De Nardo, Christine
                       M. (Editor) ; Totowa, NJ : Humana
                       Press, 2013, Chapter 8 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-62703-522-4=978-1-62703-523-1 ;
                       doi:10.1007/978-1-62703-523-1},
      abstract     = {All inflammasomes require the adapter protein apoptosis
                      associated speck-like protein containing a CARD (ASC) for
                      the activation of caspase-1. After inflammasome activation,
                      ASC assembles into a large protein complex, which is termed
                      'speck'. ASC specks can be observed as they reach a size of
                      around 1 μm and in most cells only one speck forms upon
                      inflammasome activation. Hence, ASC speck formation can be
                      used as a simple upstream readout for inflammasome
                      activation. Here, we describe a method for analyzing
                      inflammasome activation by ASC speck visualization. First,
                      we describe the generation of a clonal inflammasome reporter
                      macrophage cell line overexpressing fluorescently tagged
                      ASC. We then discuss stimulation conditions and the
                      microscopic evaluation of ASC speck formation.},
      cin          = {AG Latz},
      ddc          = {570},
      cid          = {I:(DE-2719)1013024},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:23852599},
      doi          = {10.1007/978-1-62703-523-1_8},
      url          = {https://pub.dzne.de/record/164601},
}