guest ::
| Home > Publications Database > ASC Speck Formation as a Readout for Inflammasome Activation > print |
| 001 | 164601 | ||
| 005 | 20230726100520.0 | ||
| 020 | _ | _ | |a 978-1-62703-522-4 (print) |
| 020 | _ | _ | |a 978-1-62703-523-1 (electronic) |
| 024 | 7 | _ | |a 10.1007/978-1-62703-523-1_8 |2 doi |
| 024 | 7 | _ | |a 1064-3745 |2 ISSN |
| 024 | 7 | _ | |a 1940-6029 |2 ISSN |
| 024 | 7 | _ | |a altmetric:22261264 |2 altmetric |
| 024 | 7 | _ | |a pmid:23852599 |2 pmid |
| 037 | _ | _ | |a DZNE-2022-01144 |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a De Nardo, Christine M. |b 0 |e Editor |
| 245 | _ | _ | |a ASC Speck Formation as a Readout for Inflammasome Activation |
| 260 | _ | _ | |a Totowa, NJ |c 2013 |b Humana Press |
| 295 | 1 | 0 | |a The Inflammasome / De Nardo, Christine M. (Editor) ; Totowa, NJ : Humana Press, 2013, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-62703-522-4=978-1-62703-523-1 ; doi:10.1007/978-1-62703-523-1 |
| 300 | _ | _ | |a 91 - 101 |
| 336 | 7 | _ | |a BOOK_CHAPTER |2 ORCID |
| 336 | 7 | _ | |a Book Section |0 7 |2 EndNote |
| 336 | 7 | _ | |a bookPart |2 DRIVER |
| 336 | 7 | _ | |a INBOOK |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Book chapter |2 DataCite |
| 336 | 7 | _ | |a Contribution to a book |b contb |m contb |0 PUB:(DE-HGF)7 |s 1654678582_2870 |2 PUB:(DE-HGF) |
| 490 | 0 | _ | |a Methods in Molecular Biology |v 1040 |
| 520 | _ | _ | |a All inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed 'speck'. ASC specks can be observed as they reach a size of around 1 μm and in most cells only one speck forms upon inflammasome activation. Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC speck visualization. First, we describe the generation of a clonal inflammasome reporter macrophage cell line overexpressing fluorescently tagged ASC. We then discuss stimulation conditions and the microscopic evaluation of ASC speck formation. |
| 536 | _ | _ | |a 899 - ohne Topic (POF4-899) |0 G:(DE-HGF)POF4-899 |c POF4-899 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef Book Series, Journals: pub.dzne.de |
| 700 | 1 | _ | |a Latz, Eicke |b 1 |e Editor |
| 700 | 1 | _ | |a Stutz, Andrea |b 2 |
| 700 | 1 | _ | |a Horvath, Gabor L. |b 3 |
| 700 | 1 | _ | |a Monks, Brian G. |b 4 |
| 700 | 1 | _ | |a Latz, Eicke |0 P:(DE-2719)2000062 |b 5 |e Last author |u dzne |
| 773 | _ | _ | |a 10.1007/978-1-62703-523-1_8 |
| 909 | C | O | |o oai:pub.dzne.de:164601 |p VDB |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 5 |6 P:(DE-2719)2000062 |
| 913 | 1 | _ | |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |1 G:(DE-HGF)POF4-890 |0 G:(DE-HGF)POF4-899 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-800 |4 G:(DE-HGF)POF |v ohne Topic |x 0 |
| 914 | 1 | _ | |y 2013 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2020-09-11 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2020-09-11 |
| 920 | 1 | _ | |0 I:(DE-2719)1013024 |k AG Latz |l Innate Immunity in Neurodegeneration |x 0 |
| 980 | _ | _ | |a contb |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-2719)1013024 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|