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000164838 0247_ $$2doi$$a10.1091/mbc.E21-11-0585
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000164838 1001_ $$0P:(DE-2719)2812073$$aHansen, Jan N$$b0$$udzne
000164838 245__ $$aMotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia.
000164838 260__ $$aBethesda, Md.$$bAmerican Society for Cell Biology$$c2022
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000164838 520__ $$aMicroglia are the primary resident innate immune cells of the central nervous system (CNS). They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia. 
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000164838 650_2 $$2MeSH$$aCell Movement: physiology
000164838 650_2 $$2MeSH$$aMicroglia: metabolism
000164838 7001_ $$0P:(DE-2719)2813026$$aBrückner, Matthias$$b1$$udzne
000164838 7001_ $$0P:(DE-2719)2812560$$aPietrowski, Marie J$$b2$$udzne
000164838 7001_ $$aJikeli, Jan F$$b3
000164838 7001_ $$0P:(DE-2719)2812070$$aPlescher, Monika$$b4$$udzne
000164838 7001_ $$0P:(DE-2719)2812062$$aBeckert, Hannes$$b5$$udzne
000164838 7001_ $$aSchnaars, Mareike$$b6
000164838 7001_ $$aFülle, Lorenz$$b7
000164838 7001_ $$aReitmeier, Katharina$$b8
000164838 7001_ $$aLangmann, Thomas$$b9
000164838 7001_ $$aFörster, Irmgard$$b10
000164838 7001_ $$aBoche, Delphine$$b11
000164838 7001_ $$0P:(DE-2719)2810273$$aPetzold, Gabor C$$b12$$udzne
000164838 7001_ $$0P:(DE-2719)2812038$$aHalle, Annett$$b13$$eLast author$$udzne
000164838 773__ $$0PERI:(DE-600)1474922-1$$a10.1091/mbc.E21-11-0585$$n11$$par99$$tMolecular biology of the cell$$v33$$x1044-2030$$y2022
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