Journal Article

DZNE-2022-01282

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia.

Hansen, J. N.DZNE* ; Brückner, M.Extern* ; Pietrowski, M. J.DZNE* ; Jikeli, J. F. ; Plescher, M.DZNE* ; Beckert, H.DZNE* ; Schnaars, M. ; Fülle, L. ; Reitmeier, K. ; Langmann, T. ; Förster, I. ; Boche, D. ; Petzold, G. C.DZNE* ; Halle, A. (Last author)DZNE*

2022
American Society for Cell Biology Bethesda, Md.

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Please use a persistent id in citations: doi:10.1091/mbc.E21-11-0585

Abstract: Microglia are the primary resident innate immune cells of the central nervous system (CNS). They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia.

Keyword(s): Cell Movement: physiology (MeSH) ; Microglia: metabolism (MeSH)

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Contributing Institute(s):

1. Microglia and Neuroinflammation (AG Halle)
2. Aging and neurodegeneration (AG Bano)
3. Neurovascular Diseases (AG Petzold)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)
2. 351 - Brain Function (POF4-351) (POF4-351)
3. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2022

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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