000165568 001__ 165568
000165568 005__ 20240112171940.0
000165568 020__ $$a978-1-0716-2654-2 (print)
000165568 020__ $$a978-1-0716-2655-9 (electronic)
000165568 0247_ $$2doi$$a10.1007/978-1-0716-2655-9_8
000165568 0247_ $$2pmid$$apmid:36399269
000165568 0247_ $$2ISSN$$a1064-3745
000165568 0247_ $$2ISSN$$a1940-6029
000165568 0247_ $$2altmetric$$aaltmetric:138628551
000165568 037__ $$aDZNE-2022-01708
000165568 041__ $$aEnglish
000165568 082__ $$a570
000165568 1001_ $$0P:(DE-2719)2812737$$aSiddiqui, Tohid$$b0$$eFirst author
000165568 245__ $$aThree-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.
000165568 260__ $$aNew York, NY$$bSpringer US$$c2023
000165568 29510 $$aAlzheimer’s Disease / Chun, Jerold (Editor) ; New York, NY : Springer US, 2023, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2654-2=978-1-0716-2655-9 ; doi:10.1007/978-1-0716-2655-9
000165568 300__ $$a159 - 170
000165568 3367_ $$2ORCID$$aBOOK_CHAPTER
000165568 3367_ $$07$$2EndNote$$aBook Section
000165568 3367_ $$2DRIVER$$abookPart
000165568 3367_ $$2BibTeX$$aINBOOK
000165568 3367_ $$2DataCite$$aOutput Types/Book chapter
000165568 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1694166854_31831
000165568 4900_ $$aMethods in Molecular Biology$$v2561
000165568 520__ $$aIn this chapter, we present the methodology currently used in our laboratory to generate a starPEG-MMP (starPEG)- and heparin maleimide HM06 (heparin)-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer's disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysiological activity, tissue-specific extracellular matrix (ECM) deposition, and neurotransmitter responsiveness. When treated with amyloid beta-42 (Aβ42) peptides, this system recapitulates many of the pathological effects of AD, including reduced neural stem cell proliferation, impaired neuronal network formation, dystrophic axonal ends, synaptic loss, failure to deposit ECM, elevated tau hyperphosphorylation, and formation of neurofibrillary tangles. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD.
000165568 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000165568 588__ $$aDataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
000165568 650_2 $$2MeSH$$aHumans
000165568 650_2 $$2MeSH$$aAlzheimer Disease: metabolism
000165568 650_2 $$2MeSH$$aAmyloid beta-Peptides: metabolism
000165568 650_2 $$2MeSH$$aHydrogels: metabolism
000165568 650_2 $$2MeSH$$aHeparin: metabolism
000165568 650_2 $$2MeSH$$aNeurons: metabolism
000165568 650_7 $$2Other$$aAlzheimer’s disease
000165568 650_7 $$2Other$$a3D
000165568 650_7 $$2Other$$aAlzheimer’s disease
000165568 650_7 $$2Other$$aAmyloid beta-42 (Aβ42)
000165568 650_7 $$2Other$$aBiohybrid hydrogel cell culture
000165568 650_7 $$2Other$$aHeparin
000165568 650_7 $$2Other$$aHuman neural development
000165568 650_7 $$2Other$$aPrimary human astrocytes
000165568 650_7 $$2Other$$aStarPEG
000165568 650_7 $$2Other$$aiPSC-derived neural stem cells
000165568 650_7 $$2NLM Chemicals$$aAmyloid beta-Peptides
000165568 650_7 $$2NLM Chemicals$$aHydrogels
000165568 650_7 $$09005-49-6$$2NLM Chemicals$$aHeparin
000165568 650_7 $$2Other$$aAmyloid beta-42 (Aβ42)
000165568 7001_ $$0P:(DE-2719)2813050$$aCelikkaya, Hilal$$b1
000165568 7001_ $$0P:(DE-2719)9002487$$aAtasavum, Zeynep Tansu$$b2$$udzne
000165568 7001_ $$0P:(DE-2719)2812472$$aPopova, Stanislava$$b3
000165568 7001_ $$aFreudenberg, Uwe$$b4
000165568 7001_ $$aWerner, Carsten$$b5
000165568 7001_ $$0P:(DE-2719)2811030$$aKizil, Caghan$$b6$$eLast author
000165568 773__ $$a10.1007/978-1-0716-2655-9_8
000165568 8564_ $$uhttps://pub.dzne.de/record/165568/files/DZNE-2022-01708_Restricted.pdf
000165568 8564_ $$uhttps://pub.dzne.de/record/165568/files/DZNE-2022-01708_Restricted.pdf?subformat=pdfa$$xpdfa
000165568 909CO $$ooai:pub.dzne.de:165568$$pVDB
000165568 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812737$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000165568 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2813050$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000165568 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9002487$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000165568 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812472$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000165568 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811030$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b6$$kDZNE
000165568 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000165568 9141_ $$y2023
000165568 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-09-11
000165568 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-09-11
000165568 9201_ $$0I:(DE-2719)1710007$$kAG Kizil$$lMechanisms of Induced Plasticity of the Vertebrate Brain$$x0
000165568 980__ $$acontb
000165568 980__ $$aVDB
000165568 980__ $$aI:(DE-2719)1710007
000165568 980__ $$aUNRESTRICTED