Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.

Siddiqui, Tohid; Kizil, Caghan; Celikkaya, Hilal; Werner, Carsten; Popova, Stanislava; Atasavum, Zeynep Tansu; Freudenberg, Uwe

doi:10.1007/978-1-0716-2655-9_8

Contribution to a book

DZNE-2022-01708

Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.

Siddiqui, T. (First author)DZNE* ; Celikkaya, H.DZNE* ; Atasavum, Z. T.DZNE* ; Popova, S.DZNE* ; Freudenberg, U. ; Werner, C. ; Kizil, C. (Last author)DZNE*

2023
Springer US New York, NY
ISBN: 978-1-0716-2654-2 (print), 978-1-0716-2655-9 (electronic)

Alzheimer’s Disease / Chun, Jerold (Editor) ; New York, NY : Springer US, 2023, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2654-2=978-1-0716-2655-9 ; doi:10.1007/978-1-0716-2655-9 New York, NY : Springer US, Methods in Molecular Biology 2561, 159 - 170 (2023) [10.1007/978-1-0716-2655-9_8]

This record in other databases:

Please use a persistent id in citations: doi:10.1007/978-1-0716-2655-9_8

Abstract: In this chapter, we present the methodology currently used in our laboratory to generate a starPEG-MMP (starPEG)- and heparin maleimide HM06 (heparin)-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer's disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysiological activity, tissue-specific extracellular matrix (ECM) deposition, and neurotransmitter responsiveness. When treated with amyloid beta-42 (Aβ42) peptides, this system recapitulates many of the pathological effects of AD, including reduced neural stem cell proliferation, impaired neuronal network formation, dystrophic axonal ends, synaptic loss, failure to deposit ECM, elevated tau hyperphosphorylation, and formation of neurofibrillary tangles. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD.

Keyword(s): Humans (MeSH) ; Alzheimer Disease: metabolism (MeSH) ; Amyloid beta-Peptides: metabolism (MeSH) ; Hydrogels: metabolism (MeSH) ; Heparin: metabolism (MeSH) ; Neurons: metabolism (MeSH) ; Alzheimer’s disease ; 3D ; Alzheimer’s disease ; Amyloid beta-42 (Aβ42) ; Biohybrid hydrogel cell culture ; Heparin ; Human neural development ; Primary human astrocytes ; StarPEG ; iPSC-derived neural stem cells ; Amyloid beta-Peptides ; Hydrogels ; Heparin ; Amyloid beta-42 (Aβ42)