TY  - CHAP
AU  - Siddiqui, Tohid
AU  - Celikkaya, Hilal
AU  - Atasavum, Zeynep Tansu
AU  - Popova, Stanislava
AU  - Freudenberg, Uwe
AU  - Werner, Carsten
AU  - Kizil, Caghan
TI  - Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.
VL  - 2561
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2022-01708
SN  - 978-1-0716-2654-2 (print)
T2  - Methods in Molecular Biology
SP  - 159 - 170
PY  - 2023
AB  - In this chapter, we present the methodology currently used in our laboratory to generate a starPEG-MMP (starPEG)- and heparin maleimide HM06 (heparin)-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer's disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysiological activity, tissue-specific extracellular matrix (ECM) deposition, and neurotransmitter responsiveness. When treated with amyloid beta-42 (Aβ42) peptides, this system recapitulates many of the pathological effects of AD, including reduced neural stem cell proliferation, impaired neuronal network formation, dystrophic axonal ends, synaptic loss, failure to deposit ECM, elevated tau hyperphosphorylation, and formation of neurofibrillary tangles. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD.
KW  - Humans
KW  - Alzheimer Disease: metabolism
KW  - Amyloid beta-Peptides: metabolism
KW  - Hydrogels: metabolism
KW  - Heparin: metabolism
KW  - Neurons: metabolism
KW  - Alzheimer’s disease (Other)
KW  - 3D (Other)
KW  - Alzheimer’s disease (Other)
KW  - Amyloid beta-42 (Aβ42) (Other)
KW  - Biohybrid hydrogel cell culture (Other)
KW  - Heparin (Other)
KW  - Human neural development (Other)
KW  - Primary human astrocytes (Other)
KW  - StarPEG (Other)
KW  - iPSC-derived neural stem cells (Other)
KW  - Amyloid beta-Peptides (NLM Chemicals)
KW  - Hydrogels (NLM Chemicals)
KW  - Heparin (NLM Chemicals)
KW  - Amyloid beta-42 (Aβ42) (Other)
LB  - PUB:(DE-HGF)7
C6  - pmid:36399269
DO  - DOI:10.1007/978-1-0716-2655-9_8
UR  - https://pub.dzne.de/record/165568
ER  -