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@INBOOK{Siddiqui:165568,
      author       = {Siddiqui, Tohid and Celikkaya, Hilal and Atasavum, Zeynep
                      Tansu and Popova, Stanislava and Freudenberg, Uwe and
                      Werner, Carsten and Kizil, Caghan},
      title        = {{T}hree-{D}imensional {B}iohybrid {S}tar{PEG}-{H}eparin
                      {H}ydrogel {C}ultures for {M}odeling {H}uman {N}euronal
                      {D}evelopment and {A}lzheimer's {D}isease {P}athology.},
      volume       = {2561},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {DZNE-2022-01708},
      isbn         = {978-1-0716-2654-2 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {159 - 170},
      year         = {2023},
      comment      = {Alzheimer’s Disease / Chun, Jerold (Editor) ; New York,
                      NY : Springer US, 2023, Chapter 8 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-0716-2654-2=978-1-0716-2655-9 ;
                      doi:10.1007/978-1-0716-2655-9},
      booktitle     = {Alzheimer’s Disease / Chun, Jerold
                       (Editor) ; New York, NY : Springer US,
                       2023, Chapter 8 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-2654-2=978-1-0716-2655-9 ;
                       doi:10.1007/978-1-0716-2655-9},
      abstract     = {In this chapter, we present the methodology currently used
                      in our laboratory to generate a starPEG-MMP (starPEG)- and
                      heparin maleimide HM06 (heparin)-based 3D cell culture
                      system, in a hydrogel, that can be used to study human
                      neuronal development and Alzheimer's disease (AD) pathology.
                      A 3D cell culture system can mimic the in vivo cellular
                      environment better than a 2D format, in which these cells
                      exhibit neural network formation, electrophysiological
                      activity, tissue-specific extracellular matrix (ECM)
                      deposition, and neurotransmitter responsiveness. When
                      treated with amyloid beta-42 (Aβ42) peptides, this system
                      recapitulates many of the pathological effects of AD,
                      including reduced neural stem cell proliferation, impaired
                      neuronal network formation, dystrophic axonal ends, synaptic
                      loss, failure to deposit ECM, elevated tau
                      hyperphosphorylation, and formation of neurofibrillary
                      tangles. Culturing human primary cortical astrocyte (pHA)-
                      or induced pluripotent stem cell (iPSC)-derived human neural
                      stem cells in this biohybrid hydrogel system has led to the
                      discovery of novel regulatory pathways underlying
                      neurodegenerative pathology in different phases of AD.},
      keywords     = {Humans / Alzheimer Disease: metabolism / Amyloid
                      beta-Peptides: metabolism / Hydrogels: metabolism / Heparin:
                      metabolism / Neurons: metabolism / Alzheimer’s disease
                      (Other) / 3D (Other) / Alzheimer’s disease (Other) /
                      Amyloid beta-42 (Aβ42) (Other) / Biohybrid hydrogel cell
                      culture (Other) / Heparin (Other) / Human neural development
                      (Other) / Primary human astrocytes (Other) / StarPEG (Other)
                      / iPSC-derived neural stem cells (Other) / Amyloid
                      beta-Peptides (NLM Chemicals) / Hydrogels (NLM Chemicals) /
                      Heparin (NLM Chemicals) / Amyloid beta-42 (Aβ42) (Other)},
      cin          = {AG Kizil},
      ddc          = {570},
      cid          = {I:(DE-2719)1710007},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:36399269},
      doi          = {10.1007/978-1-0716-2655-9_8},
      url          = {https://pub.dzne.de/record/165568},
}