Home > Publications Database > Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology. > print

001     165568
005     20240112171940.0
020 _ _ |a 978-1-0716-2654-2 (print)
020 _ _ |a 978-1-0716-2655-9 (electronic)
024 7 _ |a 10.1007/978-1-0716-2655-9_8
|2 doi
024 7 _ |a pmid:36399269
|2 pmid
024 7 _ |a 1064-3745
|2 ISSN
024 7 _ |a 1940-6029
|2 ISSN
024 7 _ |a altmetric:138628551
|2 altmetric
037 _ _ |a DZNE-2022-01708
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Siddiqui, Tohid
|0 P:(DE-2719)2812737
|b 0
|e First author
245 _ _ |a Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.
260 _ _ |a New York, NY
|c 2023
|b Springer US
295 1 0 |a Alzheimer’s Disease / Chun, Jerold (Editor) ; New York, NY : Springer US, 2023, Chapter 8 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2654-2=978-1-0716-2655-9 ; doi:10.1007/978-1-0716-2655-9
300 _ _ |a 159 - 170
336 7 _ |a BOOK_CHAPTER
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336 7 _ |a Book Section
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336 7 _ |a bookPart
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336 7 _ |a INBOOK
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336 7 _ |a Output Types/Book chapter
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336 7 _ |a Contribution to a book
|b contb
|m contb
|0 PUB:(DE-HGF)7
|s 1694166854_31831
|2 PUB:(DE-HGF)
490 0 _ |a Methods in Molecular Biology
|v 2561
520 _ _ |a In this chapter, we present the methodology currently used in our laboratory to generate a starPEG-MMP (starPEG)- and heparin maleimide HM06 (heparin)-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer's disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysiological activity, tissue-specific extracellular matrix (ECM) deposition, and neurotransmitter responsiveness. When treated with amyloid beta-42 (Aβ42) peptides, this system recapitulates many of the pathological effects of AD, including reduced neural stem cell proliferation, impaired neuronal network formation, dystrophic axonal ends, synaptic loss, failure to deposit ECM, elevated tau hyperphosphorylation, and formation of neurofibrillary tangles. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD.
536 _ _ |a 352 - Disease Mechanisms (POF4-352)
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588 _ _ |a Dataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Alzheimer Disease: metabolism
|2 MeSH
650 _ 2 |a Amyloid beta-Peptides: metabolism
|2 MeSH
650 _ 2 |a Hydrogels: metabolism
|2 MeSH
650 _ 2 |a Heparin: metabolism
|2 MeSH
650 _ 2 |a Neurons: metabolism
|2 MeSH
650 _ 7 |a Alzheimer’s disease
|2 Other
650 _ 7 |a 3D
|2 Other
650 _ 7 |a Alzheimer’s disease
|2 Other
650 _ 7 |a Amyloid beta-42 (Aβ42)
|2 Other
650 _ 7 |a Biohybrid hydrogel cell culture
|2 Other
650 _ 7 |a Heparin
|2 Other
650 _ 7 |a Human neural development
|2 Other
650 _ 7 |a Primary human astrocytes
|2 Other
650 _ 7 |a StarPEG
|2 Other
650 _ 7 |a iPSC-derived neural stem cells
|2 Other
650 _ 7 |a Amyloid beta-Peptides
|2 NLM Chemicals
650 _ 7 |a Hydrogels
|2 NLM Chemicals
650 _ 7 |a Heparin
|0 9005-49-6
|2 NLM Chemicals
650 _ 7 |a Amyloid beta-42 (Aβ42)
|2 Other
700 1 _ |a Celikkaya, Hilal
|0 P:(DE-2719)2813050
|b 1
700 1 _ |a Atasavum, Zeynep Tansu
|0 P:(DE-2719)9002487
|b 2
|u dzne
700 1 _ |a Popova, Stanislava
|0 P:(DE-2719)2812472
|b 3
700 1 _ |a Freudenberg, Uwe
|b 4
700 1 _ |a Werner, Carsten
|b 5
700 1 _ |a Kizil, Caghan
|0 P:(DE-2719)2811030
|b 6
|e Last author
773 _ _ |a 10.1007/978-1-0716-2655-9_8
856 4 _ |u https://pub.dzne.de/record/165568/files/DZNE-2022-01708_Restricted.pdf
856 4 _ |u https://pub.dzne.de/record/165568/files/DZNE-2022-01708_Restricted.pdf?subformat=pdfa
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909 C O |p VDB
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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913 1 _ |a DE-HGF
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|l Neurodegenerative Diseases
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|0 G:(DE-HGF)POF4-352
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|v Disease Mechanisms
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914 1 _ |y 2023
915 _ _ |a DBCoverage
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915 _ _ |a DBCoverage
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920 1 _ |0 I:(DE-2719)1710007
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980 _ _ |a contb
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1710007
980 _ _ |a UNRESTRICTED

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