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000169132 041__ $$aEnglish
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000169132 1001_ $$aPantazis, Caroline B$$b0
000169132 245__ $$aA reference human induced pluripotent stem cell line for large-scale collaborative studies.
000169132 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2022
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000169132 520__ $$aHuman induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.
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000169132 650_7 $$2Other$$aCRISPR
000169132 650_7 $$2Other$$adifferentiation
000169132 650_7 $$2Other$$aiPSC
000169132 650_7 $$2Other$$akaryotype
000169132 650_7 $$2Other$$ap53
000169132 650_7 $$2Other$$apluripotent
000169132 650_7 $$2Other$$areference
000169132 650_7 $$2Other$$asingle-cell
000169132 650_7 $$2Other$$astem cell
000169132 650_7 $$2Other$$awhole-genome
000169132 650_2 $$2MeSH$$aHumans
000169132 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells
000169132 650_2 $$2MeSH$$aCell Differentiation
000169132 650_2 $$2MeSH$$aGene Editing
000169132 650_2 $$2MeSH$$aBiological Assay
000169132 7001_ $$aYang, Andrian$$b1
000169132 7001_ $$aLara, Erika$$b2
000169132 7001_ $$aMcDonough, Justin A$$b3
000169132 7001_ $$0P:(DE-2719)2810837$$aBlauwendraat, Cornelis$$b4$$udzne
000169132 7001_ $$aPeng, Lirong$$b5
000169132 7001_ $$aOguro, Hideyuki$$b6
000169132 7001_ $$aKanaujiya, Jitendra$$b7
000169132 7001_ $$aZou, Jizhong$$b8
000169132 7001_ $$aSebesta, David$$b9
000169132 7001_ $$aPratt, Gretchen$$b10
000169132 7001_ $$aCross, Erin$$b11
000169132 7001_ $$aBlockwick, Jeffrey$$b12
000169132 7001_ $$aBuxton, Philip$$b13
000169132 7001_ $$aKinner-Bibeau, Lauren$$b14
000169132 7001_ $$aMedura, Constance$$b15
000169132 7001_ $$aTompkins, Christopher$$b16
000169132 7001_ $$aHughes, Stephen$$b17
000169132 7001_ $$aSantiana, Marianita$$b18
000169132 7001_ $$aFaghri, Faraz$$b19
000169132 7001_ $$aNalls, Mike A$$b20
000169132 7001_ $$aVitale, Daniel$$b21
000169132 7001_ $$aBallard, Shannon$$b22
000169132 7001_ $$aQi, Yue A$$b23
000169132 7001_ $$aRamos, Daniel M$$b24
000169132 7001_ $$aAnderson, Kailyn M$$b25
000169132 7001_ $$aStadler, Julia$$b26
000169132 7001_ $$aNarayan, Priyanka$$b27
000169132 7001_ $$aPapademetriou, Jason$$b28
000169132 7001_ $$aReilly, Luke$$b29
000169132 7001_ $$aNelson, Matthew P$$b30
000169132 7001_ $$aAggarwal, Sanya$$b31
000169132 7001_ $$aRosen, Leah U$$b32
000169132 7001_ $$aKirwan, Peter$$b33
000169132 7001_ $$aPisupati, Venkat$$b34
000169132 7001_ $$aCoon, Steven L$$b35
000169132 7001_ $$aScholz, Sonja W$$b36
000169132 7001_ $$aPriebe, Theresa$$b37
000169132 7001_ $$aÖttl, Miriam$$b38
000169132 7001_ $$aDong, Jian$$b39
000169132 7001_ $$aMeijer, Marieke$$b40
000169132 7001_ $$aJanssen, Lara J M$$b41
000169132 7001_ $$aLourenco, Vanessa S$$b42
000169132 7001_ $$avan der Kant, Rik$$b43
000169132 7001_ $$aCrusius, Dennis$$b44
000169132 7001_ $$0P:(DE-2719)2010112$$aPaquet, Dominik$$b45$$udzne
000169132 7001_ $$aRaulin, Ana-Caroline$$b46
000169132 7001_ $$aBu, Guojun$$b47
000169132 7001_ $$aHeld, Aaron$$b48
000169132 7001_ $$aWainger, Brian J$$b49
000169132 7001_ $$aGabriele, Rebecca M C$$b50
000169132 7001_ $$aCasey, Jackie M$$b51
000169132 7001_ $$aWray, Selina$$b52
000169132 7001_ $$aAbu-Bonsrah, Dad$$b53
000169132 7001_ $$aParish, Clare L$$b54
000169132 7001_ $$aBeccari, Melinda S$$b55
000169132 7001_ $$aCleveland, Don W$$b56
000169132 7001_ $$aLi, Emmy$$b57
000169132 7001_ $$aRose, Indigo V L$$b58
000169132 7001_ $$aKampmann, Martin$$b59
000169132 7001_ $$aCalatayud Aristoy, Carles$$b60
000169132 7001_ $$aVerstreken, Patrik$$b61
000169132 7001_ $$aHeinrich, Laurin$$b62
000169132 7001_ $$aChen, Max Y$$b63
000169132 7001_ $$aSchüle, Birgitt$$b64
000169132 7001_ $$aDou, Dan$$b65
000169132 7001_ $$aHolzbaur, Erika L F$$b66
000169132 7001_ $$aZanellati, Maria Clara$$b67
000169132 7001_ $$aBasundra, Richa$$b68
000169132 7001_ $$aDeshmukh, Mohanish$$b69
000169132 7001_ $$aCohen, Sarah$$b70
000169132 7001_ $$aKhanna, Richa$$b71
000169132 7001_ $$aRaman, Malavika$$b72
000169132 7001_ $$aNevin, Zachary S$$b73
000169132 7001_ $$aMatia, Madeline$$b74
000169132 7001_ $$aVan Lent, Jonas$$b75
000169132 7001_ $$aTimmerman, Vincent$$b76
000169132 7001_ $$aConklin, Bruce R$$b77
000169132 7001_ $$aJohnson Chase, Katherine$$b78
000169132 7001_ $$aZhang, Ke$$b79
000169132 7001_ $$aFunes, Salome$$b80
000169132 7001_ $$aBosco, Daryl A$$b81
000169132 7001_ $$0P:(DE-2719)9000542$$aErlebach, Lena$$b82$$udzne
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000169132 7001_ $$aLyu, Guochang$$b85
000169132 7001_ $$aArenas, Ernest$$b86
000169132 7001_ $$aCoccia, Elena$$b87
000169132 7001_ $$aSarrafha, Lily$$b88
000169132 7001_ $$aAhfeldt, Tim$$b89
000169132 7001_ $$aMarioni, John C$$b90
000169132 7001_ $$aSkarnes, William C$$b91
000169132 7001_ $$aCookson, Mark R$$b92
000169132 7001_ $$aWard, Michael E$$b93
000169132 7001_ $$aMerkle, Florian T$$b94
000169132 773__ $$0PERI:(DE-600)2375356-0$$a10.1016/j.stem.2022.11.004$$gVol. 29, no. 12, p. 1685 - 1702.e22$$n12$$p1685 - 1702.e22$$tCell stem cell$$v29$$x1934-5909$$y2022
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