Journal Article

DZNE-2023-00011

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A reference human induced pluripotent stem cell line for large-scale collaborative studies.

Pantazis, C. B. ; Yang, A. ; Lara, E. ; McDonough, J. A. ; Blauwendraat, C.Extern* ; Peng, L. ; Oguro, H. ; Kanaujiya, J. ; Zou, J. ; Sebesta, D. ; Pratt, G. ; Cross, E. ; Blockwick, J. ; Buxton, P. ; Kinner-Bibeau, L. ; Medura, C. ; Tompkins, C. ; Hughes, S. ; Santiana, M. ; Faghri, F. ; Nalls, M. A. ; Vitale, D. ; Ballard, S. ; Qi, Y. A. ; Ramos, D. M. ; Anderson, K. M. ; Stadler, J. ; Narayan, P. ; Papademetriou, J. ; Reilly, L. ; Nelson, M. P. ; Aggarwal, S. ; Rosen, L. U. ; Kirwan, P. ; Pisupati, V. ; Coon, S. L. ; Scholz, S. W. ; Priebe, T. ; Öttl, M. ; Dong, J. ; Meijer, M. ; Janssen, L. J. M. ; Lourenco, V. S. ; van der Kant, R. ; Crusius, D. ; Paquet, D.Extern* ; Raulin, A.-C. ; Bu, G. ; Held, A. ; Wainger, B. J. ; Gabriele, R. M. C. ; Casey, J. M. ; Wray, S. ; Abu-Bonsrah, D. ; Parish, C. L. ; Beccari, M. S. ; Cleveland, D. W. ; Li, E. ; Rose, I. V. L. ; Kampmann, M. ; Calatayud Aristoy, C. ; Verstreken, P. ; Heinrich, L. ; Chen, M. Y. ; Schüle, B. ; Dou, D. ; Holzbaur, E. L. F. ; Zanellati, M. C. ; Basundra, R. ; Deshmukh, M. ; Cohen, S. ; Khanna, R. ; Raman, M. ; Nevin, Z. S. ; Matia, M. ; Van Lent, J. ; Timmerman, V. ; Conklin, B. R. ; Johnson Chase, K. ; Zhang, K. ; Funes, S. ; Bosco, D. A. ; Erlebach, L.DZNE* ; Welzer, M.DZNE* ; Kronenberg-Versteeg, D.DZNE* ; Lyu, G. ; Arenas, E. ; Coccia, E. ; Sarrafha, L. ; Ahfeldt, T. ; Marioni, J. C. ; Skarnes, W. C. ; Cookson, M. R. ; Ward, M. E. ; Merkle, F. T.

2022
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.stem.2022.11.004

Abstract: Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.

Keyword(s): Humans (MeSH) ; Induced Pluripotent Stem Cells (MeSH) ; Cell Differentiation (MeSH) ; Gene Editing (MeSH) ; Biological Assay (MeSH) ; CRISPR ; differentiation ; iPSC ; karyotype ; p53 ; pluripotent ; reference ; single-cell ; stem cell ; whole-genome

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Contributing Institute(s):

1. Cell Biology of Neurological Diseases (AG Jucker)
2. Applied Genomics for Neurodegenerative Diseases (AG Rizzu)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)
2. 354 - Disease Prevention and Healthy Aging (POF4-354) (POF4-354)

Appears in the scientific report 2022

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Essential Science Indicators ; IF >= 25 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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