TY  - JOUR
AU  - Pantazis, Caroline B
AU  - Yang, Andrian
AU  - Lara, Erika
AU  - McDonough, Justin A
AU  - Blauwendraat, Cornelis
AU  - Peng, Lirong
AU  - Oguro, Hideyuki
AU  - Kanaujiya, Jitendra
AU  - Zou, Jizhong
AU  - Sebesta, David
AU  - Pratt, Gretchen
AU  - Cross, Erin
AU  - Blockwick, Jeffrey
AU  - Buxton, Philip
AU  - Kinner-Bibeau, Lauren
AU  - Medura, Constance
AU  - Tompkins, Christopher
AU  - Hughes, Stephen
AU  - Santiana, Marianita
AU  - Faghri, Faraz
AU  - Nalls, Mike A
AU  - Vitale, Daniel
AU  - Ballard, Shannon
AU  - Qi, Yue A
AU  - Ramos, Daniel M
AU  - Anderson, Kailyn M
AU  - Stadler, Julia
AU  - Narayan, Priyanka
AU  - Papademetriou, Jason
AU  - Reilly, Luke
AU  - Nelson, Matthew P
AU  - Aggarwal, Sanya
AU  - Rosen, Leah U
AU  - Kirwan, Peter
AU  - Pisupati, Venkat
AU  - Coon, Steven L
AU  - Scholz, Sonja W
AU  - Priebe, Theresa
AU  - Öttl, Miriam
AU  - Dong, Jian
AU  - Meijer, Marieke
AU  - Janssen, Lara J M
AU  - Lourenco, Vanessa S
AU  - van der Kant, Rik
AU  - Crusius, Dennis
AU  - Paquet, Dominik
AU  - Raulin, Ana-Caroline
AU  - Bu, Guojun
AU  - Held, Aaron
AU  - Wainger, Brian J
AU  - Gabriele, Rebecca M C
AU  - Casey, Jackie M
AU  - Wray, Selina
AU  - Abu-Bonsrah, Dad
AU  - Parish, Clare L
AU  - Beccari, Melinda S
AU  - Cleveland, Don W
AU  - Li, Emmy
AU  - Rose, Indigo V L
AU  - Kampmann, Martin
AU  - Calatayud Aristoy, Carles
AU  - Verstreken, Patrik
AU  - Heinrich, Laurin
AU  - Chen, Max Y
AU  - Schüle, Birgitt
AU  - Dou, Dan
AU  - Holzbaur, Erika L F
AU  - Zanellati, Maria Clara
AU  - Basundra, Richa
AU  - Deshmukh, Mohanish
AU  - Cohen, Sarah
AU  - Khanna, Richa
AU  - Raman, Malavika
AU  - Nevin, Zachary S
AU  - Matia, Madeline
AU  - Van Lent, Jonas
AU  - Timmerman, Vincent
AU  - Conklin, Bruce R
AU  - Johnson Chase, Katherine
AU  - Zhang, Ke
AU  - Funes, Salome
AU  - Bosco, Daryl A
AU  - Erlebach, Lena
AU  - Welzer, Marc
AU  - Kronenberg-Versteeg, Deborah
AU  - Lyu, Guochang
AU  - Arenas, Ernest
AU  - Coccia, Elena
AU  - Sarrafha, Lily
AU  - Ahfeldt, Tim
AU  - Marioni, John C
AU  - Skarnes, William C
AU  - Cookson, Mark R
AU  - Ward, Michael E
AU  - Merkle, Florian T
TI  - A reference human induced pluripotent stem cell line for large-scale collaborative studies.
JO  - Cell stem cell
VL  - 29
IS  - 12
SN  - 1934-5909
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - DZNE-2023-00011
SP  - 1685 - 1702.e22
PY  - 2022
AB  - Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.
KW  - Humans
KW  - Induced Pluripotent Stem Cells
KW  - Cell Differentiation
KW  - Gene Editing
KW  - Biological Assay
KW  - CRISPR (Other)
KW  - differentiation (Other)
KW  - iPSC (Other)
KW  - karyotype (Other)
KW  - p53 (Other)
KW  - pluripotent (Other)
KW  - reference (Other)
KW  - single-cell (Other)
KW  - stem cell (Other)
KW  - whole-genome (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36459969
C2  - pmc:PMC9782786
DO  - DOI:10.1016/j.stem.2022.11.004
UR  - https://pub.dzne.de/record/169132
ER  -