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@ARTICLE{Pantazis:169132,
author = {Pantazis, Caroline B and Yang, Andrian and Lara, Erika and
McDonough, Justin A and Blauwendraat, Cornelis and Peng,
Lirong and Oguro, Hideyuki and Kanaujiya, Jitendra and Zou,
Jizhong and Sebesta, David and Pratt, Gretchen and Cross,
Erin and Blockwick, Jeffrey and Buxton, Philip and
Kinner-Bibeau, Lauren and Medura, Constance and Tompkins,
Christopher and Hughes, Stephen and Santiana, Marianita and
Faghri, Faraz and Nalls, Mike A and Vitale, Daniel and
Ballard, Shannon and Qi, Yue A and Ramos, Daniel M and
Anderson, Kailyn M and Stadler, Julia and Narayan, Priyanka
and Papademetriou, Jason and Reilly, Luke and Nelson,
Matthew P and Aggarwal, Sanya and Rosen, Leah U and Kirwan,
Peter and Pisupati, Venkat and Coon, Steven L and Scholz,
Sonja W and Priebe, Theresa and Öttl, Miriam and Dong, Jian
and Meijer, Marieke and Janssen, Lara J M and Lourenco,
Vanessa S and van der Kant, Rik and Crusius, Dennis and
Paquet, Dominik and Raulin, Ana-Caroline and Bu, Guojun and
Held, Aaron and Wainger, Brian J and Gabriele, Rebecca M C
and Casey, Jackie M and Wray, Selina and Abu-Bonsrah, Dad
and Parish, Clare L and Beccari, Melinda S and Cleveland,
Don W and Li, Emmy and Rose, Indigo V L and Kampmann, Martin
and Calatayud Aristoy, Carles and Verstreken, Patrik and
Heinrich, Laurin and Chen, Max Y and Schüle, Birgitt and
Dou, Dan and Holzbaur, Erika L F and Zanellati, Maria Clara
and Basundra, Richa and Deshmukh, Mohanish and Cohen, Sarah
and Khanna, Richa and Raman, Malavika and Nevin, Zachary S
and Matia, Madeline and Van Lent, Jonas and Timmerman,
Vincent and Conklin, Bruce R and Johnson Chase, Katherine
and Zhang, Ke and Funes, Salome and Bosco, Daryl A and
Erlebach, Lena and Welzer, Marc and Kronenberg-Versteeg,
Deborah and Lyu, Guochang and Arenas, Ernest and Coccia,
Elena and Sarrafha, Lily and Ahfeldt, Tim and Marioni, John
C and Skarnes, William C and Cookson, Mark R and Ward,
Michael E and Merkle, Florian T},
title = {{A} reference human induced pluripotent stem cell line for
large-scale collaborative studies.},
journal = {Cell stem cell},
volume = {29},
number = {12},
issn = {1934-5909},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {DZNE-2023-00011},
pages = {1685 - 1702.e22},
year = {2022},
abstract = {Human induced pluripotent stem cell (iPSC) lines are a
powerful tool for studying development and disease, but the
considerable phenotypic variation between lines makes it
challenging to replicate key findings and integrate data
across research groups. To address this issue, we sub-cloned
candidate human iPSC lines and deeply characterized their
genetic properties using whole genome sequencing, their
genomic stability upon CRISPR-Cas9-based gene editing, and
their phenotypic properties including differentiation to
commonly used cell types. These studies identified KOLF2.1J
as an all-around well-performing iPSC line. We then shared
KOLF2.1J with groups around the world who tested its
performance in head-to-head comparisons with their own
preferred iPSC lines across a diverse range of
differentiation protocols and functional assays. On the
strength of these findings, we have made KOLF2.1J and its
gene-edited derivative clones readily accessible to promote
the standardization required for large-scale collaborative
science in the stem cell field.},
keywords = {Humans / Induced Pluripotent Stem Cells / Cell
Differentiation / Gene Editing / Biological Assay / CRISPR
(Other) / differentiation (Other) / iPSC (Other) / karyotype
(Other) / p53 (Other) / pluripotent (Other) / reference
(Other) / single-cell (Other) / stem cell (Other) /
whole-genome (Other)},
cin = {AG Jucker / AG Rizzu},
ddc = {570},
cid = {I:(DE-2719)1210001 / I:(DE-2719)1210009},
pnm = {352 - Disease Mechanisms (POF4-352) / 354 - Disease
Prevention and Healthy Aging (POF4-354)},
pid = {G:(DE-HGF)POF4-352 / G:(DE-HGF)POF4-354},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36459969},
pmc = {pmc:PMC9782786},
doi = {10.1016/j.stem.2022.11.004},
url = {https://pub.dzne.de/record/169132},
}