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000169134 1001_ $$aKubitschke, Martin$$b0
000169134 245__ $$aNext generation genetically encoded fluorescent sensors for serotonin.
000169134 260__ $$a[London]$$bNature Publishing Group UK$$c2022
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000169134 520__ $$aWe developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.
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000169134 650_7 $$0333DO1RDJY$$2NLM Chemicals$$aSerotonin
000169134 650_2 $$2MeSH$$aSerotonin
000169134 7001_ $$0P:(DE-2719)2811495$$aMüller, Monika$$b1$$udzne
000169134 7001_ $$00000-0001-6238-7982$$aWallhorn, Lutz$$b2
000169134 7001_ $$00000-0001-6255-0276$$aPulin, Mauro$$b3
000169134 7001_ $$0P:(DE-2719)2811044$$aMittag, Manuel$$b4$$udzne
000169134 7001_ $$aPollok, Stefan$$b5
000169134 7001_ $$00000-0001-6673-198X$$aZiebarth, Tim$$b6
000169134 7001_ $$aBremshey, Svenja$$b7
000169134 7001_ $$00000-0002-4528-9421$$aGerdey, Jill$$b8
000169134 7001_ $$aClaussen, Kristin Carolin$$b9
000169134 7001_ $$aRenken, Kim$$b10
000169134 7001_ $$aGroß, Juliana$$b11
000169134 7001_ $$aGneiße, Pascal$$b12
000169134 7001_ $$aMeyer, Niklas$$b13
000169134 7001_ $$00000-0003-0893-9349$$aWiegert, J Simon$$b14
000169134 7001_ $$00000-0003-0802-7278$$aReiner, Andreas$$b15
000169134 7001_ $$0P:(DE-2719)2679991$$aFuhrmann, Martin$$b16$$udzne
000169134 7001_ $$00000-0002-1541-2388$$aMasseck, Olivia Andrea$$b17
000169134 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-022-35200-w$$gVol. 13, no. 1, p. 7525$$n1$$p7525$$tNature Communications$$v13$$x2041-1723$$y2022
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