Journal Article

DZNE-2023-00013

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Next generation genetically encoded fluorescent sensors for serotonin.

Kubitschke, M. ; Müller, M.DZNE* ; Wallhorn, L. ; Pulin, M. ; Mittag, M.DZNE* ; Pollok, S. ; Ziebarth, T. ; Bremshey, S. ; Gerdey, J. ; Claussen, K. C. ; Renken, K. ; Groß, J. ; Gneiße, P. ; Meyer, N. ; Wiegert, J. S. ; Reiner, A. ; Fuhrmann, M.DZNE* ; Masseck, O. A.

2022
Nature Publishing Group UK [London]

This record in other databases:    

Please use a persistent id in citations: doi:10.1038/s41467-022-35200-w

Abstract: We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

Keyword(s): Serotonin (MeSH) ; Serotonin

---

Contributing Institute(s):

1. Neuroimmunology and Imaging (AG Fuhrmann)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2022

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---