TY  - JOUR
AU  - Kubitschke, Martin
AU  - Müller, Monika
AU  - Wallhorn, Lutz
AU  - Pulin, Mauro
AU  - Mittag, Manuel
AU  - Pollok, Stefan
AU  - Ziebarth, Tim
AU  - Bremshey, Svenja
AU  - Gerdey, Jill
AU  - Claussen, Kristin Carolin
AU  - Renken, Kim
AU  - Groß, Juliana
AU  - Gneiße, Pascal
AU  - Meyer, Niklas
AU  - Wiegert, J Simon
AU  - Reiner, Andreas
AU  - Fuhrmann, Martin
AU  - Masseck, Olivia Andrea
TI  - Next generation genetically encoded fluorescent sensors for serotonin.
JO  - Nature Communications
VL  - 13
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - DZNE-2023-00013
SP  - 7525
PY  - 2022
AB  - We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.
KW  - Serotonin
KW  - Serotonin (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:36473867
C2  - pmc:PMC9726753
DO  - DOI:10.1038/s41467-022-35200-w
UR  - https://pub.dzne.de/record/169134
ER  -