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@ARTICLE{Kubitschke:169134,
      author       = {Kubitschke, Martin and Müller, Monika and Wallhorn, Lutz
                      and Pulin, Mauro and Mittag, Manuel and Pollok, Stefan and
                      Ziebarth, Tim and Bremshey, Svenja and Gerdey, Jill and
                      Claussen, Kristin Carolin and Renken, Kim and Groß, Juliana
                      and Gneiße, Pascal and Meyer, Niklas and Wiegert, J Simon
                      and Reiner, Andreas and Fuhrmann, Martin and Masseck, Olivia
                      Andrea},
      title        = {{N}ext generation genetically encoded fluorescent sensors
                      for serotonin.},
      journal      = {Nature Communications},
      volume       = {13},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {DZNE-2023-00013},
      pages        = {7525},
      year         = {2022},
      abstract     = {We developed a family of genetically encoded serotonin
                      (5-HT) sensors (sDarken) on the basis of the native 5-HT1A
                      receptor and circularly permuted GFP. sDarken 5-HT sensors
                      are bright in the unbound state and diminish their
                      fluorescence upon binding of 5-HT. Sensor variants with
                      different affinities for serotonin were engineered to
                      increase the versatility in imaging of serotonin dynamics.
                      Experiments in vitro and in vivo showed the feasibility of
                      imaging serotonin dynamics with high temporal and spatial
                      resolution. As demonstrated here, the designed sensors show
                      excellent membrane expression, have high specificity and a
                      superior signal-to-noise ratio, detect the endogenous
                      release of serotonin and are suitable for two-photon in vivo
                      imaging.},
      keywords     = {Serotonin / Serotonin (NLM Chemicals)},
      cin          = {AG Fuhrmann},
      ddc          = {500},
      cid          = {I:(DE-2719)1011004},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36473867},
      pmc          = {pmc:PMC9726753},
      doi          = {10.1038/s41467-022-35200-w},
      url          = {https://pub.dzne.de/record/169134},
}