000248112 001__ 248112
000248112 005__ 20240610153937.0
000248112 037__ $$aDZNE-2023-00216
000248112 041__ $$aEnglish
000248112 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$eFirst author
000248112 245__ $$aDataset: Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
000248112 260__ $$bPRoteomics IDEntifications Database$$c2021
000248112 3367_ $$2BibTeX$$aMISC
000248112 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1718026763_5322
000248112 3367_ $$026$$2EndNote$$aChart or Table
000248112 3367_ $$2DataCite$$aDataset
000248112 3367_ $$2ORCID$$aDATA_SET
000248112 3367_ $$2DINI$$aResearchData
000248112 520__ $$aA disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. ADAM10 was recently proposed to function as six distinct scissors with different substrates, depending on its association with one of six regulatory tetraspanins, termed TspanC8s. However, it remains unclear whether ADAM10 function is critically dependent on a TspanC8 partner. To address this, we generated the first monoclonal antibodies to Tspan15 as a model TspanC8. These were used to show that ADAM10 is the principal Tspan15-interacting protein, that Tspan15 expression requires ADAM10 in cell lines and primary cells, and that a synthetic ADAM10/Tspan15 fusion protein is a functional scissor. Together these findings suggest that ADAM10 exists as an ADAM10/TspanC8 scissor complex.
000248112 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000248112 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x1
000248112 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$eLast author
000248112 7870_ $$0DZNE-2020-01462$$aKoo, Chek Ziu et.al.$$dBethesda, Md. : Soc., 2020$$iRelatedTo$$r$$tThe tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
000248112 8564_ $$uhttps://www.ebi.ac.uk/pride/archive/projects/PXD016508
000248112 909CO $$ooai:pub.dzne.de:248112$$pVDB
000248112 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000248112 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000248112 9131_ $$0G:(DE-HGF)POF3-342$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vDisease Mechanisms and Model Systems$$x0
000248112 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x1
000248112 9141_ $$y2021
000248112 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000248112 980__ $$adataset
000248112 980__ $$aVDB
000248112 980__ $$aI:(DE-2719)1110006
000248112 980__ $$aUNRESTRICTED