Dataset

DZNE-2023-00216

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex

Müller, S. A. (First author)DZNE* ; Lichtenthaler, S. (Last author)DZNE*

2021
PRoteomics IDEntifications Database

Abstract: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. ADAM10 was recently proposed to function as six distinct scissors with different substrates, depending on its association with one of six regulatory tetraspanins, termed TspanC8s. However, it remains unclear whether ADAM10 function is critically dependent on a TspanC8 partner. To address this, we generated the first monoclonal antibodies to Tspan15 as a model TspanC8. These were used to show that ADAM10 is the principal Tspan15-interacting protein, that Tspan15 expression requires ADAM10 in cell lines and primary cells, and that a synthetic ADAM10/Tspan15 fusion protein is a functional scissor. Together these findings suggest that ADAM10 exists as an ADAM10/TspanC8 scissor complex.

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Contributing Institute(s):

1. Neuroproteomics (AG Lichtenthaler)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)
2. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2021

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Koo, C. Z. ; Harrison, N. ; Noy, P. J. ; Szyroka, J. ; Matthews, A. L. ; Hsia, H.-E.DZNE* ; Müller, S. A.DZNE* ; Tüshaus, J.DZNE* ; Goulding, J. ; Willis, K. ; Apicella, C. ; Cragoe, B. ; Davis, E. ; Keles, M. ; Malinova, A. ; McFarlane, T. A. ; Morrison, P. R. ; Nguyen, H. T. H. ; Sykes, M. C. ; Ahmed, H. ; Di Maio, A. ; Seipold, L. ; Saftig, P. ; Cull, E. ; Pliotas, C. ; Rubinstein, E. ; Poulter, N. S. ; Briddon, S. J. ; Holliday, N. D. ; Lichtenthaler, S.DZNE* ; Tomlinson, M. G. (Corresponding author)
The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
The journal of biological chemistry 295(36), 12822 - 12839 (2020) [10.1074/jbc.RA120.012601]2020   Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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