%0 Chart or Table
%A Gloeckner, Christian Johannes
%T Dataset: Identification of mitotic substrates of phospho-regulated USP9X
%I PRoteomics IDEntifications Database
%M DZNE-2023-00238
%D 2020
%X We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732) and subsequently identified USP9X as a specific CDC14B target (PXD012733). To investigate relevant mitotic substrates of phospho-regulated USP9X, we next performed a Stable isotope labeling by amino acids in cell culture (SILAC)-based screen in which ubiquitylated proteins were purified from control or USP9X depleted HEK 293T cells that were either asynchronous or synchronized in mitosis. Stable expression of recombinant His-tagged ubiquitin has been used for selective enrichment of ubiquitinylated proteins in both conditions.
%F PUB:(DE-HGF)32
%9 Dataset
%U https://pub.dzne.de/record/249923