Dataset

DZNE-2023-00238

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Identification of mitotic substrates of phospho-regulated USP9X

Gloeckner, C. J. (Last author)DZNE*

2020
PRoteomics IDEntifications Database

Abstract: We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732) and subsequently identified USP9X as a specific CDC14B target (PXD012733). To investigate relevant mitotic substrates of phospho-regulated USP9X, we next performed a Stable isotope labeling by amino acids in cell culture (SILAC)-based screen in which ubiquitylated proteins were purified from control or USP9X depleted HEK 293T cells that were either asynchronous or synchronized in mitosis. Stable expression of recombinant His-tagged ubiquitin has been used for selective enrichment of ubiquitinylated proteins in both conditions.

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Contributing Institute(s):

1. Functional Neuroproteomics and Translational Biomarkers in Neurodegenerative Diseases (AG Gloeckner)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2020

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Dietachmayr, M. ; Rathakrishnan, A. ; Karpiuk, O. ; von Zweydorf, F.DZNE* ; Engleitner, T. ; Fernández-Sáiz, V. ; Schenk, P. ; Ueffing, M. ; Rad, R. ; Eilers, M. ; Gloeckner, C. J.DZNE* ; Clemm von Hohenberg, K. (Corresponding author) ; Bassermann, F.
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Nature Communications 11(1), 1268 (2020) [10.1038/s41467-020-15059-5]2020   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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